539576

PKA, Catalytic Subunit, Bovine Heart

• Synonyme(s) : cAMP-Dependent Protein Kinase Catalytic Subunit, Protein Kinase A, Catalytic Subunit
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.77

Propriétés

• Source biologique: bovine heart
• Niveau de qualité: 100
• Pureté: ≥90% (SDS-PAGE)
• Forme: liquid
• Activité spécifique: ≥20,000 units/μg protein
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; avoid repeated freeze/thaw cycles
• Technique(s): activity assay: suitable
• Adéquation: suitable for molecular biology
• Conditions d'expédition: wet ice
• Température de stockage: −70°C

Description

Description générale

Research area: Cell Signaling

Native PKA catalytic subunit purified from bovine heart. Protein Kinase A (PKA) is a serine protein kinase that targets serine and threonine residues.

Actions biochimiques/physiologiques

Protein kinases are crucial for integrating signaling networks within eukaryotic cells. It plays an essential role in regulating proliferation and fate determination by influencing Hedgehog signaling transduction. Upon binding of cAMP to the PKA regulatory subunits, the catalytic subunit of PKA dissociates from the PKA holoenzyme and proceeds to phosphorylate target proteins at serine and/or threonine within the recognition motif, Arg-Arg-X-Ser*-X. The purified monomeric catalytic subunit is capable of activation without the need for cAMP and is well-suited for in vitro studies of PKA phosphorylation with purified substrates or when introduced into intact cells or cell extracts.

Conditionnement

Please refer to vial label for lot-specific concentration.

Avertissement

Toxicity: Standard Handling (A)

Définition de l'unité

One unit is defined as the amount of enzyme required to incorporate 1 pmol phosphate into Kemptide substrate (Cat. No. 05-23-4900) per min at 30°C.

Forme physique

In 350 mM K₂HPO₄, 100 µM DTT, pH 6.8.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Autres remarques

Corbin, J.D., et al. 1988. Methods Enzymol159, 74.
Rannels, S.R., et al 1983. Methods Enzymol99, 55.
Witt, J.J. and Roskoski, R. Jr. 1975. Anal Biochem.66, 253.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable