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P0497

Sigma-Aldrich

Anti-PMP70 antibody produced in rabbit

enhanced validation

affinity isolated antibody, buffered aqueous solution

Synonyme(s) :

Anti-70 kDa Peroxisomal membrane Protein, Anti-ABCD3, Anti-PXMP1

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About This Item

Numéro MDL:
MFCD01865555
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Poids mol.

antigen 70 kDa

Espèces réactives

human, mouse, rat

Validation améliorée

independent
Learn more about Antibody Enhanced Validation

Technique(s)

immunoprecipitation (IP): 1-2 μg using RIPA extract (0.5 mg) from human HepG2 cells
indirect immunofluorescence: 4-8 μg/mL using mouse NIH3T3 cells
western blot (chemiluminescent): 0.5-1 μg/mL using whole extracts of rat PC12 cells or rat kidney extract or rat liver extract

Numéro d'accès UniProt

P28288

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... ABCD3(5825)
mouse ... Abcd3(19299)
rat ... Abcd3(25270)

Catégories apparentées

Description générale

The 70 kDa peroxisomal membrane protein (PMP70) is a major component of peroxisomal membranes. The peroxisome is a multifunctional single-membrane organelle associated with eukaryotic cells. PMP70 belongs to the ATP binding cassette (ABC) transporter superfamily.

Immunogène

synthetic peptide corresponding to amino acid residues 644-659 of rat PMP70 with N-terminal added cysteine, conjugated to KLH. The corresponding sequence is identical in mouse and differs by one amino acid in human.

Application

Anti-PMP70 antibody produced in rabbit has been used in:
  • immunocytochemistry
  • immunostaining
  • immunofluorescence
  • western blotting
  • immunoprecipitation

Actions biochimiques/physiologiques

PMP70 participates in the metabolic transport of long and very long fatty acids into peroxisomes. PMP70 interacts and forms a stable complex with the adrenoleukodystrophy protein(ALDP), and also with several other peroxisomal proteins. ATP binding/hydrolysis by PMP70 and ALDL and their phosphorylation are involved in the regulation of fatty acid transport into peroxisomes. Mutations in the PMP70 (PXMP1) gene may cause a subset of Zellweger syndrome, an autosomal recessive disorder and is characterized by defects in import mechanism for peroxisomal matrix enzymes.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

The pestivirus N terminal protease Npro redistributes to mitochondria and peroxisomes suggesting new sites for regulation of IRF3 by Npro
Jefferson M, et al.
Testing, 9(2), e88838-e88838 (2014)
Kyle A Lyman et al.
The Journal of biological chemistry, 292(43), 17718-17730 (2017-09-10)
Tetratricopeptide repeat (TPR) domains are ubiquitous structural motifs that mediate protein-protein interactions. For example, the TPR domains in the peroxisomal import receptor PEX5 enable binding to a range of type 1 peroxisomal targeting signal motifs. A homolog of PEX5, tetratricopeptide
Pioglitazone significantly prevented decreased rate of neural differentiation of mouse embryonic stem cells which was reduced by Pex11beta knock-down
Esmaeili M, et al.
Neuroscience, 312, 35-47 (2016)
Direct membrane association drives mitochondrial fission by the Parkinson disease-associated protein alpha-synuclein
Nakamura K, et al.
Test, 286(23), 20710-20726 (2011)
Mutations in the 70K peroxisomal membrane protein gene in Zellweger syndrome
Gartner J, et al.
Nature Genetics, 1(1), 16-16 (1992)
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Articles

Organelle Isolation

The isolation of subcellular fractions by centrifugation is a commonly used technique and is widely applicable across multiple cell and tissue types. Because organelles differ in their size, shape, and density, centrifugation can be easily employed to separate and purify organelle fractions from gently homogenized samples.

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