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P0050

Sigma-Aldrich

Anti-Peroxiredoxin 6 (N-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonyme(s) :

Anti-1-Cys peroxiredoxin; acidic calcium-independent phospholipase A2, Anti-Antioxidant protein 2, Anti-NSGPx, Anti-Non-selenium glutathione peroxidase, AOP2, Anti-P29, Anti-PRDX6, Anti-PRX, Anti-aiPLA2

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Poids mol.

antigen ~25 kDa

Espèces réactives

human, mouse, rat

Conditionnement

antibody small pack of 25 μL

Concentration

~1.0 mg/mL

Technique(s)

immunoprecipitation (IP): 5-10 μg using lysate of mouse brain
western blot: 2-5 μg/mL using whole extract of human HeLa cells
western blot: 5-10 μg/mL using whole extract of rat liver

Numéro d'accès UniProt

P30041

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... PRDX6(9588)
mouse ... Prdx6(11758)
rat ... Prdx6(94167)

Description générale

Peroxiredoxin 6 (1-Cys peroxiredoxin), is a member of the thiol-specific antioxidant peroxiredoxin family. Six mammalian peroxiredoxins have been identified. Peroxiredoxin 6 is the only one that contains a single redox-active cysteine and uses glutathione to catalyze the reduction of H2O2 and other organic peroxides. It is expressed in all tissues with the highest levels in the lung.

Application

The antibody has been used in several immunochemical techniques including immunoblotting and immunoprecipitation.

Actions biochimiques/physiologiques

Peroxiredoxins are peroxidases, with the use of reducing equivalents provided by thiol-containing proteins that reduce hydrogen peroxide (H2O2) to water and alkyl hydroperoxides to alcohol. Peroxiredoxin 6 is a bifunctional enzyme with peroxidase and phospholipase A2 activities. Overexpression of peroxiredoxin 6 in cells protects them against oxidative damage, whereas knockdown of this enzyme results in oxidative stress and apoptosis. The phospholipase A2 activity plays an important role in surfactant homeostasis. Peroxiredoxin 6 is a major antioxidant enzyme which functions in antioxidant defense and lung phospholipid metabolism.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

1-Cys Peroxiredoxin, a Bifunctional Enzyme with Glutathione Peroxidase and Phospholipase A2 Activities
Chen JW, et al.
The Journal of biological chemistry, 275(37), 28421-28427 (2000)
Hyun Ae Woo et al.
The Journal of biological chemistry, 278(48), 47361-47364 (2003-10-16)
We previously suggested that oxidation of the active site cysteine of peroxiredoxin (Prx) I or Prx II to cysteine sulfinic acid in H2O2-treated cells is reversible (Woo, H. A., Chae, H. Z., Hwang, S. C., Yang, K.-S., Kang, S. W.
Yefim Manevich et al.
Free radical biology & medicine, 38(11), 1422-1432 (2005-05-14)
Peroxiredoxin 6 (Prdx6), a bifunctional 25-kDa protein with both GSH peroxidase and phospholipase A2 activities, is the only mammalian 1-Cys member of the peroxiredoxin superfamily and is expressed in all major organs, with a particularly high level in lung. Prdx6
C McDonald et al.
International journal of oncology, 45(1), 219-226 (2014-05-03)
Peroxiredoxin (Prdx) proteins are thiol-specific antioxidants that protect cells from oxidative stress in many normal and disease states. There are six Prdx proteins expressed in mammals, each with a characteristic tissue expression, subcellular distribution and substrate specificity. Recent studies have
Ran Liu et al.
Molecular neurobiology, 50(3), 1035-1048 (2014-05-06)
Following spinal cord injury (SCI), limit spontaneous functional recovery often emerged. However, the neuronal mechanisms associated with this phenomenon still remains obscure. By using proteomics analysis, endoplasmic reticulum protein 29 (ERp29) was discovered to increase in the motor cortexes of

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