Accéder au contenu
Merck
Toutes les photos(1)

Documents

N7885

Sigma-Aldrich

Neuraminidase from Vibrio cholerae

Type III, buffered aqueous solution, 0.2 μm filtered, 1-5 units/mg protein (Lowry, using NAN-lactose)

Synonyme(s) :

Acyl-neuraminyl Hydrolase, Receptor-destroying enzyme, Sialidase

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(1)

About This Item

Numéro CAS:
9001-67-6
Numéro de classification (Commission des enzymes):
3.2.1.18 (BRENDA, IUBMB)
Numéro CE :
232-624-6
Numéro MDL:
MFCD00131711
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54

Niveau de qualité

300

Type

Type III

Forme

buffered aqueous solution

Activité spécifique

1-5 units/mg protein (Lowry, using NAN-lactose)

Poids mol.

83 kDa

Application(s)

diagnostic assay manufacturing

Activité étrangère

Protease and NAN-aldolase, present

Température de stockage

2-8°C

Vous recherchez des produits similaires ? Visite Guide de comparaison des produits

Description générale

Neuraminidase enzymes are glycoside hydrolase enzymes that catalyze hydrolysis of terminal sialic acid residues. The most well-known are the viral nearamidases, which promote influenza virus release.

Application

Neuraminidase from Vibrio cholerae has been used in a study to assess its role in the binding and uptake of cholera toxin by susceptible cells. It has also been used in a study to investigate the preparation of a sodium substrate and its use in a fluorometric assay of neuraminidase.
Neurminidase is used as a cell-surface probe for glycoconjugate distribution and in substrate specificity studies.

Actions biochimiques/physiologiques

Vibrio cholerae neuraminidase has been shown to promote cholera toxin infection by binding the toxin and aiding in its uptake by cells.

Qualité

Preservative free.

Définition de l'unité

One unit will liberate 1.0 μmole of N-acetylneuraminic acid per min at pH 5.0 at 37 °C using NAN-lactose or bovine submaxillary mucin, unless otherwise specified. Prices based on units using NAN-lactose as substrate.

Forme physique

Aqueous solution, pH 5.5, containing 0.15 M NaCl and 4 mM CaCl2.

Notes préparatoires

Chromatographically purified.

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H334

Conseils de prudence

P261 - P284 - P501

Classification des risques

Resp. Sens. 1

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Jai Woong Seo et al.
Nature communications, 11(1), 2102-2102 (2020-05-02)
Adeno-associated viruses (AAVs) are typically single-stranded deoxyribonucleic acid (ssDNA) encapsulated within 25-nm protein capsids. Recently, tissue-specific AAV capsids (e.g. PHP.eB) have been shown to enhance brain delivery in rodents via the LY6A receptor on brain endothelial cells. Here, we create
J E Galen et al.
Infection and immunity, 60(2), 406-415 (1992-02-01)
Vibrio cholerae neuraminidase (NANase) is hypothesized to act synergistically with cholera toxin (CT) and increase the severity of a secretory response by increasing the binding and penetration of CT to enterocytes. To test this hypothesis, the NANase gene (nanH) from
Fluorometric assay of neuraminidase with a sodium (4-methylumbelliferyl-alpha-D-N-acetylneuraminate) substrate.
M Potier et al.
Analytical biochemistry, 94(2), 287-296 (1979-04-15)
Mikhail N Matrosovich et al.
Methods in molecular biology (Clifton, N.J.), 865, 71-94 (2012-04-25)
Influenza virus attachment to sialic acid-containing molecules on the cell surface initiates the infection. The spectrum of functional receptors on target cells and decoy receptors on cells and epithelial mucus varies substantially between animal species leading to variations in the
Lenka Horníková et al.
Current protocols in microbiology, 38, 14F-14F (2015-08-04)
Mouse polyomavirus (MPyV) is a member of the Polyomaviridae family, which comprises non-enveloped tumorigenic viruses infecting various vertebrates including humans and causing different pathogenic responses in the infected organisms. Despite the variations in host tropism and pathogenicity, the structure of
Afficher tous les articles scientifiques apparentés

Protocoles

Enzymatic Assay of Neuraminidase

Enzymatic Assay of Neuraminidase applies to products that have a specification for neuraminidase content by enzymatic determination.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique