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N3001

Sigma-Aldrich

Neuraminidase from Clostridium perfringens (C. welchii)

Type VI, lyophilized powder, 6-15 units/mg protein (using 4MU-NANA), 2-10 units/mg protein (mucin)

Synonyme(s) :

Acyl-neuraminyl Hydrolase, Receptor-destroying enzyme, Sialidase

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About This Item

Numéro CAS:
9001-67-6
Numéro de classification (Commission des enzymes):
3.2.1.18 (BRENDA, IUBMB)
Numéro CE :
232-624-6
Numéro MDL:
MFCD00131711
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54

Source biologique

Clostridium perfringens str. 13

Niveau de qualité

200

Type

Type VI

Forme

lyophilized powder

Activité spécifique

2-10 units/mg protein (mucin)
6-15 units/mg protein (using 4MU-NANA)

Composition

Protein, ≥50% biuret

Température de stockage

−20°C

Informations sur le gène

Clostridium perfringens str. 13 ... nanI(988807)

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Description générale

Neuraminidase enzymes are hydrolase enzymes that promote influenza virus release from infected cells and facilitate virus spread.

Application

Neuraminidase from Clostridium perfringens (C. welchii) has been used in a study to assess a glycoprotein faction suitable for use as a substrate in preparation assays. It has also been used in a study to investigate the action of an epsilion-toxin on MDCK cells.

Actions biochimiques/physiologiques

Neuraminidase cleavage of sialic acid groups has been used to study recognition by antibodies of glycoprotein structures. The use of neuraminidase in the estimation of N-acetylneuraminic acid was compared favorably to two other methods.
Neuraminidase from Clostridium perfringens reduces the viability of human leukemic myeloblasts and attenuates their ability to activate lymphocytes.
Neuraminidases are used to cleave terminal N-acetyl neuraminic acid (sialic acid) from a variety of glycoproteins. The enzyme from Clostridium perfringens cleaves terminal sialic acid residues which are α-2,3- α-2,6- or α-2,8-linked to Gal, GlcNac, GalNAc, AcNeu, GlcNeu, oligosaccharides, glycolipids or glycoproteins. The relative rate of cleavage decreases in the order: α-2-3 > α-2-6 . α-2-8. Neuraminidase from C. perfringens cleaves α-2-3 linked sialic acid residues most efficiently, compared to A. ureafaciens, (Sigma N3642) which preferentially cleaves α-2-6 linked residues.
The use of neuraminidase to remove sialic acid residues from glycoproteins on cell surfaces has been frequently reported. Generally, procedures have indicated using neuraminidase in PBS at 37°C for 30 minutes, followed by several washings with PBS. Treatment of tissue sections with neuraminidase at much lower concentrations require longer incubation: for 1-4 U/mL in 0.1 M acetate buffer pH 4.2-5, from 2 to 20 hours at 37 °C.

Notes préparatoires

Chromatographically purified from Type V (N 2876)

Remarque sur l'analyse

Package sizes based on 4MU-NANA units
Package sizes based on the 4MU-NANA units

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H334

Conseils de prudence

P261 - P284 - P501

Classification des risques

Resp. Sens. 1

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

C Ogier et al.
Biomedicine / [publiee pour l'A.A.I.C.I.G.], 31(9-10), 250-252 (1979-12-01)
The effect of increasing concentrations of Cl. Perfringens neuraminidase and of pH on the dye exclusion ability and lymphocyte stimulating capacity of leukemic myeloblasts was studied. The higher the neuraminidase concentration, or the lower the pH was, the more myeloblasts
Ana León-Rodríguez et al.
Scientific reports, 12(1), 11581-11581 (2022-07-09)
Short-term behavioral alterations are associated with infection and aid the recovery from sickness. However, concerns have raised that sustained behavioral disturbances after acute neuroinflammation could relate to neurological diseases in the long run. We aimed to explore medium- and long-term
L Petit et al.
Journal of bacteriology, 179(20), 6480-6487 (1997-10-23)
Epsilon-toxin is produced by Clostridium perfringens types B and D and is responsible for a rapidly fatal enterotoxemia in animals, which is characterized by edema in several organs due to an increase in blood vessel permeability. The Madin-Darby canine kidney
A G Fraser et al.
Journal of medical microbiology, 8(2), 235-249 (1975-05-01)
A glycoprotein fraction (fraction VII) suitable for use as a substrate in assays of microbial neuraminidase was prepared from pooled human plasma. It is pasteurised during preparation to eliminate the risk of transmission of serum hepatitis. This results in polymerisation
Gangliosides: structure, isolation, and analysis.
R W Ledeen et al.
Methods in enzymology, 83, 139-191 (1982-01-01)
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Protocoles

Enzymatic Assay of Neuraminidase

Enzymatic Assay of Neuraminidase applies to products that have a specification for neuraminidase content by enzymatic determination.

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