MAK172
Proteasome 20S Activity Assay Kit
sufficient for 100 fluorometric tests
Synonyme(s) :
20S Proteasome Detection Kit
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About This Item
Produits recommandés
Utilisation
sufficient for 100 fluorometric tests
Méthode de détection
fluorometric
Maladie(s) pertinente(s)
endocrinological disorders, diabetes; neurological disorders; cancer
Température de stockage
−20°C
Catégories apparentées
Description générale
Protein degradation occurs most commonly through two major mechanisms, lysosomal degradation and proteasome mediated degradation. Lysosomal degradation occurs through proteolytic enzyme activity and is fairly non-specific. Proteasomal degradation is targeted for a specific protein through the ubiquitination of the target protein. The target protein is typically poly-ubiquitinated and activates the 26S proteasome toward proteolysis. The proteasome 26S is the most common form of the proteasome and is an ATP-dependent proteolytic complex, which contains one 20S (700-kDa) core particle structure and two 19S (700-kDa) regulatory caps.
The proteasome is responsible for the recycling of proteins and maintenance of the balance of protein synthesis and degradation. The proteasome is involved with apoptosis, DNA repair, endocytosis, and cell cycle and has been implicated in disease states such as certain types of cancer, diabetes mellitus, and Alzheimer′s disease among others making it the target for drug discovery investigations.
The proteasome is responsible for the recycling of proteins and maintenance of the balance of protein synthesis and degradation. The proteasome is involved with apoptosis, DNA repair, endocytosis, and cell cycle and has been implicated in disease states such as certain types of cancer, diabetes mellitus, and Alzheimer′s disease among others making it the target for drug discovery investigations.
Caractéristiques et avantages
Compatible with high-throughput handling systems.
Adéquation
Suitable for measuring the chymotrypsin-like protease activity associated with the proteasome complex either in cultured cells or cell lysates.
Principe
The fluorometric proteasome 20S assay kit is a homogeneous fluorescent assay that measures the chymotrypsin-like protease activity associated with the proteasome complex in cultured cells. This kit uses LLVY-R110 as a fluorogenic indicator for proteasome activities. Cleavage of LLVY-R110 by proteasome generates strongly green fluorescent R110 that is monitored fluorimetrically at 520-530 nm with excitation at 480-500 nm. The assay is robust, and can be readily adapted for high-throughput assays to evaluate the proteasome activities or screen the inhibitors in cultured cells or in solution. The assay can be performed in a convenient 96-well and 384-well fluorescence microtiter-plate format.
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
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Javier Quero et al.
Antioxidants (Basel, Switzerland), 10(2) (2021-02-11)
The application of plant extracts for therapeutic purposes has been used in traditional medicine because plants contain bioactive compounds with beneficial properties for health. Currently, the use of these compounds that are rich in polyphenols for the treatment and prevention
The proteasome: Overview of structure and functions
Tanaka K
Proceedings of the Japan Academy. Series B, Physical and Biological Sciences, 85(1), 12-36 (2009)
Mahamud-Ur Rashid et al.
Journal of virology, 96(5), e0199021-e0199021 (2022-01-13)
Influenza A virus (IAV), an obligatory intracellular parasite, uses host cellular molecules to complete its replication cycle and suppress immune responses. Proteasome subunit alpha type 2 (PSMA2) is a cellular protein highly expressed in IAV-infected human lung epithelial A549 cells.
The Cell: A Molecular Approach (2000)
Qingtian Zhu et al.
Biochemical and biophysical research communications, 505(1), 126-133 (2018-09-22)
The proteasome is involved in the activation of NF-κB and can regulate the progression of inflammatory diseases. However, the role of proteasome in acute pancreatitis (AP) has not been demonstrated. In this study, we first observed that the protein level
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