M4159

Methylene Blue hydrate

suitable for nucleic acid staining, BioReagent

• Synonyme(s) : 3,7-Bis(dimethylamino)phenothiazin-5-ium chloride xhydrate, Methylene Blue monohydrate, Methylene blue, Nucleic acid staining reagent
• Formule empirique (notation de Hill): C₁₆H₁₈ClN₃S · xH₂O
• Numéro CAS: 122965-43-9
• Poids moléculaire : 319.85 (anhydrous basis)
• Numéro Beilstein : 3599847
• Numéro CE : 200-515-2
• Numéro MDL: MFCD00150006
• Code UNSPSC : 12352200
• ID de substance PubChem : 57654386
• Nomenclature NACRES : NA.52

Propriétés

• Qualité: for molecular biology
• Niveau de qualité: 100
• Gamme de produits: BioReagent
• Forme: powder
• Technique(s): nucleic acid detection: suitable
• Solubilité: water: 1 mg/mL (dark blue)
• Adéquation: suitable for nucleic acid staining
• Température de stockage: room temp
• Chaîne SMILES: O.[Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C
• InChI: 1S/C16H18N3S.ClH.H2O/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;;/h5-10H,1-4H3;1H;1H2/q+1;;/p-1
• Clé InChI: WQVSELLRAGBDLX-UHFFFAOYSA-M

Description

Description générale

Methylene blue is a heterocyclic aromatic chemical, appearing as a dark green solid powder, changing to blue in aqueous solution. It is used as a stain in many different histology procedures, as well as in molecular biology laboratories.

Application

Methylene Blue hydrate is suitable for use as a nucleic acid stain for agarose and polyacrylamide gel electrophoresis (PAGE). It was used to stain 4T1 tumor cells to study metastasis of tumor cells using clonogenic assay. Methylene blue hydrate crystals were encapsulated in apoferritin nanocages for photodynamic therapy against tumor cells.

Caractéristiques et avantages

• Less hazardous than staining with ethidium bromide
• Viewable in visible light
• Fast and easy to use

Informations sur la sécurité

• Pictogrammes: GHS07
• Mention d'avertissement: Warning
• Mentions de danger: H302
• Classification des risques: Acute Tox. 4 Oral
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: dust mask type N95 (US), Eyeshields, Gloves