T6146

Trypan Blue

powder, BioReagent, suitable for cell culture

• Synonyme(s) : Direct blue 14, Niagara blue reagent, blue live/dead cell assay
• Formule linéaire : C₃₄H₂₄N₆O₁₄S₄Na₄
• Numéro CAS: 72-57-1
• Poids moléculaire : 960.81
• Numéro C.I. (Colour Index): 23850
• Beilstein: 4360496
• Numéro CE : 200-786-7
• Numéro MDL: MFCD00003969
• Code UNSPSC : 12352207
• ID de substance PubChem : 57654686
• Nomenclature NACRES : NA.75

Propriétés

• Gamme de produits: BioReagent
• Niveau de qualité: 300
• Forme: powder
• Composition: Dye content, ~40%
• Conditionnement: glass bottle of 100 g; glass bottle of 25 g; glass bottle of 5 g
• Conditions de stockage: dry at room temperature
• Technique(s): cell culture | mammalian: suitable; tissue processing: suitable
• Pf: >300 °C (lit.)
• Solubilité: H₂O: 10 mg/mL
• Application(s): cell analysis
• Chaîne SMILES: [Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4
• InChI: 1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4
• Clé InChI: GLNADSQYFUSGOU-UHFFFAOYSA-J
• Informations sur le gène: human ... PTPN1(5770) , PTPRF(5792)

Description

Description générale

The trypan blue dye is an azo dye, derived from toluidine. It is soluble in water and has a molecular weight of 961 g/mol.

Application

Trypan blue has been used:

• in cell viability assay
• to stain plant tissue in order to to visualize cell death

Actions biochimiques/physiologiques

The trypan blue dye is useful to differentiate the dead and live cells based on the cell′s ability to take up or exclude the dye. A viable cell is assumed to possess an intact cell membrane and hence excludes this dye and the cytoplasm appears clear. While on the other hand non-viable cells shows a blue cytoplasm.

Notes préparatoires

To make a cell viability solution, add the T6146 Trypan Blue powder to a clean and dry glass or plastic container of appropriate capacity. Add a suitable solvent, such as sterile distilled water, to the container with the Trypan Blue powder. Stir the solution vigorously using a stir bar or a glass rod until the powder has completely dissolved. A 0.4% solution is a common concentration for typical cell viability assessment. For example, to make 100 mL of a 0.4% solution, add 400 milligrams (0.4 g) to 99.6 mL solvent.

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H350
• Conseils de prudence: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Classification des risques: Carc. 1B
• Code de la classe de stockage: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Classe de danger pour l'eau (WGK): WGK 3
• Équipement de protection individuelle: Eyeshields, Gloves, type P3 (EN 143) respirator cartridges