L1293

Anti-Lamin A (C-terminal) antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonyme(s) : Anti-LAMA, Anti-LMN1, Anti-LMNA, Anti-Lamin A/C
• Numéro MDL: MFCD01633588
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Conjugué: unconjugated
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen ~70 kDa
• Espèces réactives: rat, human, mouse
• Validation améliorée: independent; Learn more about Antibody Enhanced Validation
• Concentration: ~1 mg/mL
• Technique(s): indirect immunofluorescence: 1-2 μg/mL using human HeLa, rat NRK, and mouse 3T3 cells; western blot: 0.1-0.2 μg/mL using human HeLa nuclear extract
• Numéro d'accès UniProt: P02545
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... LMNA(4000) ; mouse ... Lmna(16905) ; rat ... Lmna(60374)

Description

Description générale

Lamin is a structural protein of the nuclear lamina. Lamin A is a type A lamin encoded by the LMNA gene. Lamin A contains 664 amino acids and is expressed in most somatic cells. It contains α-helical rod domain to enable assembly into filaments, a nuclear localization sequence, and a carboxy-terminal CAAX box isoprenylation sequence for nuclear membrane targeting.

Immunogène

synthetic peptide corresponding to amino acids 598-611 of human lamin A with a C-terminal added cysteine, conjugated to KLH. The corresponding sequence differs by one amino acid in rat, three amino acids in mouse, and by a gap of one amino acid in both rat and mouse lamin A.

Application

Anti-Lamin A (C-terminal) antibody is suitable for use in western blot (0.1-0.2 μg/mL) using HeLa nuclear extract. This antibody can also be used in indirect immunofluorescence (1-2 μg/mL) using HeLa cells, rat NRK and mouse 3T3 cells. Additionally, anti-Lamin A (C-terminal) antibodies are suitable for use in immunoblotting (approx. 70 kDa). Cleaved fragments of lamin A may form additional bands at 45-50 kDa.

Anti-Lamin A (C-terminal) antibody produced in rabbit has been used in:

• western blotting
• immunofluorescence microscopy
• immunohistochemistry

Actions biochimiques/physiologiques

Lamin A cut into a 47kDa fragment that facilitates chromatin condensation and nuclear degradation during cell death

Mutations in lamin A and C have been linked to a variety of rare human diseases including muscular dystrophy, lipodystrophy, cardiomyopathy, neuropathy, and progeroid syndromes (collectively termed laminopathies) and to premature aging (Hutchinson-Gilford progeria syndrome).

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)