G4040
Anti-Gα i3 antibody produced in rabbit
whole antiserum, buffered aqueous solution
Synonyme(s) :
Anti-87U6, Anti-ARCND1, Anti-HG1A
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About This Item
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Conjugué
unconjugated
Forme d'anticorps
whole antiserum
Type de produit anticorps
primary antibodies
Clone
polyclonal
Forme
buffered aqueous solution
Poids mol.
antigen ~40 kDa
Espèces réactives
mouse, human, rat, hamster
Technique(s)
immunoprecipitation (IP): suitable
western blot: 1:1,000-1:2,000 using solubilized mouse and rat brain membrane lysates, and mouse 3T3 cell lysates
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... GNAI3(2773)
mouse ... Gnai3(14679)
rat ... Gnai3(25643)
Description générale
G-proteins are membrane associated heterotrimeric proteins that are comprised of α-, β-, and λ-subunits. These proteins play a key role in signal transduction pathways that regulate cell′s response to several hormones, neuromodulators and ligands.
Spécificité
Recognizes Gαi3 and Gα0 G-proteins.
Immunogène
synthetic peptide corresponding to the C-terminal region of Gαi3.
Application
Anti-Gα i3 antibody produced in rabbit is suitable for immunoprecipitation at a working antibody amount of 4μL using solubilized mouse brain membrane lysates and immunoblotting at a working dilution of 1:2000 using solubilized mouse and rat brain membrane lysates, and mouse 3T3 cell lysates.
Actions biochimiques/physiologiques
Gi α 3 is a G-protein subunit that is involved in many signal transduction pathways including the mediation of EGF-induced PLC-γ activation and Ca+2 mobilization in hepatocytes. The α-subunit contains a guanine-binding domain that is in its inactive state when it is occupied by GDP. Upon activation, GDP is replaced with GTP, causing the dissociation of the α-subunit from the βλ-subunit complex. This enables the Gα-GTP complex to bind to and regulate specific signaling pathways. GTP is then hydrolyzed, allowing for re-association of the α-subunit with the βλ-subunit complex.
Forme physique
Solution of whole antiserum diluted in PBS, pH 7.4.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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Andrew Forge et al.
PLoS genetics, 13(3), e1006692-e1006692 (2017-03-28)
Behavioural anomalies suggesting an inner ear disorder were observed in a colony of transgenic mice. Affected animals were profoundly deaf. Severe hair bundle defects were identified in all outer and inner hair cells (OHC, IHC) in the cochlea and in
L J Yang et al.
The Journal of biological chemistry, 266(33), 22451-22458 (1991-11-25)
Treatment of rat hepatocytes with epidermal growth factor (EGF) produced an enhanced tyrosine phosphorylation of the EGF receptor and phospholipase C-gamma (PLC-gamma) in conjunction with the mobilization of Ca2+. Approximately 30% of the total PLC-gamma was tyrosine-phosphorylated with a maximum
T M Wilkie et al.
Society of General Physiologists series, 49, 249-270 (1994-01-01)
G protein-mediated signal transduction systems have been identified in a diverse group of eukaryotic organisms, including yeast, plants, Dictyostelium and animals. G protein signaling components have been identified in many of these organisms, from the seven transmembrane domain receptors to
Theresa M Cabrera-Vera et al.
Endocrine reviews, 24(6), 765-781 (2003-12-13)
In multicellular organisms from Caenorhabditis elegans to Homo sapiens, the maintenance of homeostasis is dependent on the continual flow and processing of information through a complex network of cells. Moreover, in order for the organism to respond to an ever-changing
Zakia A Abdelhamed et al.
Disease models & mechanisms, 8(6), 527-541 (2015-06-04)
Ciliopathies are a group of developmental disorders that manifest with multi-organ anomalies. Mutations in TMEM67 (MKS3) cause a range of human ciliopathies, including Meckel-Gruber and Joubert syndromes. In this study we describe multi-organ developmental abnormalities in the Tmem67(tm1Dgen/H1) knockout mouse
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