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F9890

Sigma-Aldrich

Anti-Human IgG4−FITC antibody, Mouse monoclonal

clone HP-6025, purified from hybridoma cell culture

Synonyme(s) :

Monoclonal Anti-Human IgG4

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

Source biologique

mouse

Niveau de qualité

300

Conjugué

FITC conjugate

Forme d'anticorps

purified from hybridoma cell culture

Type de produit anticorps

secondary antibodies

Clone

HP-6025, monoclonal

Forme

buffered aqueous solution

Conditions de stockage

protect from light

Technique(s)

dot immunobinding: 1:64 using using a 4-8 μg dot of human IgG4

Isotype

IgG1

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

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Catégories apparentées

Description générale

IgG4 (Immunoglobulin G4) is produced by T helper 2 cytokines.

Spécificité

Monoclonal Anti-Human IgG4 is specific for the IgG4 subclass and nonreactive with IgG1, IgG2 and IgG3 in an ELISA.

Application

Monoclonal Anti-Human IgG4-FITC antibody produced in mouse has been used in immunohistofluorescence and immunofluorescence assay.
The IUIS/WHO2 study singled out this monoclonal antibody as one of the most widely applicable IgG4 specific monoclonal antibodies. Monoclonal Anti-Human IgG4 may be used for the identification of the IgG4 subclass by various immunoassays including: ELISA, Imprint Immunofixation (IIF), Immunofluorometric Assay (IFMA), Hemagglutination (HA), Hemagglutination Inhibition (HAI), Particle Counting Immunoassay (PACIA), and detection of cytoplasmic IgG.

Actions biochimiques/physiologiques

IgG4 (Immunoglobulin G4) antibody has reduced affinity for C1q (first component of complement q) and hence its anti-inflammatory activity differs from that of the other IgG subclasses. IgG4 antibodies are known to actively interchange Fab arms with another molecule, resulting in recombined antibodies with two different binding specificities. This exchange is considered as an important biological mechanism that contributes to its anti-inflammatory activity. IgG4 antibodies are normally noninflammatory, but autoreactive IgG4 antibodies are known to be associated with Immunoglobulin G4 (IgG4)-related disease (IgG4-RD), characterized by infiltrating lymphoplasmacytic cells and elevated serum IgG4.

Forme physique

Solution in 0.01 M phosphate buffer, pH 8.0, containing 1% inactivated bovine serum albumin and 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

An immunofluorescence test for phospholipase-A2-receptor antibodies and its clinical usefulness in patients with membranous glomerulonephritis.
Hoxha E, et al.
Nephrology, Dialysis, and Transplantation, 26(8), 2526-2532 (2011)
Characterization of a subtype of autoimmune encephalitis with anti?contactin-associated protein-like 2 antibodies in the cerebrospinal fluid, prominent limbic symptoms, and seizures.
Joubert B, et al.
JAMA Neurology, 73(9), 1115-1124 (2016)
What is IgG4 ? A review of the biology of a unique immunoglobulin subtype.
Nirula A, et al.
Current Opinion in Rheumatology, 23(1), 119-124 (2011)
J D Isaacs et al.
Clinical and experimental immunology, 106(3), 427-433 (1996-12-01)
It is traditionally held that human IgG4 MoAbs should not deplete target cells in vivo, as this isotype is inactive in a number of in vitro assays that measure effector function. We have previously challenged this dogma, and the current
IgG4-related disease.
Mahajan V S, et al.
Annual Review of Pathology, 9, 315-347 (2014)
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