F2756

Fluorescein di(β-D-galactopyranoside)

• Synonyme(s) : FDG
• Formule empirique (notation de Hill) : C₃₂H₃₂O₁₅
• Numéro CAS: 17817-20-8
• Poids moléculaire : 656.59
• Numéro MDL: MFCD00077344
• Code UNSPSC : 12352201
• ID de substance PubChem : 24894829
• Nomenclature NACRES : NC.07

Propriétés

• Essai: ≥98% (HPLC)
• Forme: powder
• Solubilité: methanol: 20 mg/mL, clear, yellow
• Température de stockage: −20°C
• Chaîne SMILES: OC[C@H]1O[C@@H](Oc2ccc3c(Oc4cc(O[C@@H]5O[C@H](CO)[C@H](O)[C@H](O)[C@H]5O)ccc4C36OC(=O)c7ccccc67)c2)[C@H](O)[C@@H](O)[C@H]1O
• InChI: 1S/C32H32O15/c33-11-21-23(35)25(37)27(39)30(45-21)42-13-5-7-17-19(9-13)44-20-10-14(43-31-28(40)26(38)24(36)22(12-34)46-31)6-8-18(20)32(17)16-4-2-1-3-15(16)29(41)47-32/h1-10,21-28,30-31,33-40H,11-12H2/t21-,22-,23+,24+,25+,26+,27-,28-,30-,31-/m1/s1
• Clé InChI: ZTOBILYWTYHOJB-WBCGDKOGSA-N

Description

Application

Fluorescein di(β-D-galactopyranoside) (FDG) has been used for the detection of gene expression via FACS (fluorescence-activated cell sorting).

Fluorescein di(β-D-galactopyranoside) has been used as a substrate in β-galactosidase assay.

Actions biochimiques/physiologiques

Fluorescein di (β-D-galactopyranoside) (FDG) results in fluorescence after being hydrolyzed to fluorescein by β-galactosidase. It gives fluorescence proportional to the enzymatic activity. This molecule is mainly required for the detection of gene expression in transfected cells when using β-galactosidase (Lac Z) as a reporter gene.

Substrats

Fluorogenic substrate for β-galactosidase

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)