E2886
ExtrAvidin®−Peroxidase
buffered aqueous solution
Synonyme(s) :
ExtrAvidin HRP
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About This Item
Produits recommandés
Conjugué
peroxidase conjugate
Niveau de qualité
Forme
buffered aqueous solution
Technique(s)
direct ELISA: 1:2,000
dot blot: 1:4,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
Conditions d'expédition
wet ice
Température de stockage
2-8°C
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Spécificité
The product is found to be reactive with Anti-Avidin, Catalog Number A5170, and non-reactive with Anti-Streptavidin, Catalog Number S6390, by immunoelectrophoresis.
Application
ExtrAvidin®-Peroxidase is suitable for the following applications:
- Dot blot at a minimum dilution of 1:4,000
- Immunohistochemistry (using formalin-fixed, paraffin-embedded sections at a dilution of 1:100)
- Direct ELISA at a minimum dilution of 1:2,000
- Immunoprecipitation and Immunobloting
- Ra2I solid-phase binding assays
- Southwestern blotting
- Western blotting
- Immunocytochemistry
Actions biochimiques/physiologiques
Avidin is a tetrameric or dimeric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians and deposited in the whites of their eggs. It consists of four high affinity binding sites for biotin. ExtrAvidin® is prepared from egg white avidin. It is a modified form of affinity purified avidin with high specific activity of avidin and low background staining of streptavidin. It is a biotin binding protein produced by the bacteria Streptomyces avidinii. ExtrAvidin-Peroxidase may be used in conjunction with biotin in different techniques.
Forme physique
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT
Notes préparatoires
Affinity purified protein
Stockage et stabilité
Store at 2-8 °C. Do Not Freeze. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Informations légales
ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Skin Sens. 1
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Protocoles
Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.
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