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DUO92004

Sigma-Aldrich

Sonde PLA® in situ DuolinkDuolink® anti-souris MINUS

Affinity purified Donkey anti-Mouse IgG (H+L)

Synonyme(s) :

in situ Proximity Ligation Assay reagent, Protein Protein Interaction Assay reagent

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About This Item

Code UNSPSC :
12352200
Nomenclature NACRES :
NA.32

Source biologique

donkey (polyclonal)

Niveau de qualité

200

Forme d'anticorps

affinity purified immunoglobulin (secondary antibody)

Type de produit anticorps

primary antibodies

Gamme de produits

Duolink®

Espèces réactives

mouse

Technique(s)

immunofluorescence: suitable
proximity ligation assay: suitable

Adéquation

suitable for brightfield
suitable for fluorescence

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Catégories apparentées

Description générale

La sonde PLA® MINUS anti-souris in situ Duolink® contient de l′IgG (H+L) d′âne anti-souris purifiée par affinité, qui réagit avec l′ensemble de la molécule d′IgG de souris et également avec les chaînes légères d′autres immunoglobulines de souris.

Application

Duolink®proximity ligation assay(PLA®) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.

This product can be applied to both the Duolink® In Situ Fluorescence Protocol and the Duolink® In Situ Brightfield Protocol depending on the detection reagents used.

Visit our Duolink® PLA Resource Center for information on how to run a Duolink® experiment, applications, troubleshooting, and more.

To perform a complete Duolink® PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. Other necessary reagents include a pair of PLA probes from different species (one PLUS and one MINUS), detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.HRP is also available for brightfield detection.

Specificity
PLA probe anti-Mouse reacts with whole molecule Mouse IgG and the light chains of other Mouse immunoglobulin′s. The PLA probe anti-Mouse may cross-react with Rat antibodies, but has minimal cross reactivity with bovine, chicken, goat, guinea pig, Syrian hamster, horse, human, rabbit, and sheep serum proteins. A PLUS probe of a different species must be used simultaneously with this product. See our Product Selection Guide for more information.

Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink®PLA in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.

Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink® projects

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Specificity
PLA probe anti-Mouse reacts with whole molecule Mouse IgG and the light chains of other Mouse immunoglobulin?s. The PLA probe anti-Mouse may cross-react with Rat antibodies, but has minimal cross reactivity with bovine, chicken, goat, guinea pig, Syrian hamster, horse, human, rabbit, and sheep serum proteins. A PLUS probe of a different species must be used simultaneously with this product. See our Product Selection Guide for more information.

Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink®PLA in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.

Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink® projects

View full Duolink® product list

Caractéristiques et avantages

  • Aucune surexpression ou manipulation génétique nécessaire
  • Grande spécificité (moins de faux positifs)
  • Sensibilité à une seule molécule du fait de l′amplification par cercle roulant
  • Quantification relative possible
  • Aucun équipement spécial requis
  • Plus rapide et plus simple que la technique FRET
  • Exactitude accrue par rapport à co-IP
  • Résultats prêts à être publiés

Composants

Ce produit est constitué des éléments suivants :
  • 5x Sonde PLA MINUS anti-souris - anticorps secondaire d′âne anti-souris conjugué à un oligonucléotide MINUS
  • 1x Solution de blocage - réactif permettant de bloquer l′échantillon
  • 1x Diluant pour anticorps - pour la dilution des sondes PLA et des anticorps primaires
Se reporter à la fiche technique pour de plus amples informations.

Notes préparatoires

Stockage et stabilité : conserver la sonde PLA MINUS anti-souris entre 2 et 8 °C. Ne pas conserver la solution diluée de sonde PLA MINUS anti-souris.

Autres remarques

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Informations légales

Duolink is a registered trademark of Merck KGaA, Darmstadt, Germany
PLA is a registered trademark of Merck KGaA, Darmstadt, Germany

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Pictogrammes

Exclamation markEnvironment
GHS07,GHS09

Mention d'avertissement

Warning

Mentions de danger

H317 - H411

Classification des risques

Aquatic Chronic 2 - Skin Sens. 1

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Novel function of ceramide for regulation of mitochondrial ATP release in astrocytes.
Kong J N, et al.
Journal of Lipid Research, M081877-M081877 (2018)
Interaction of the Mouse Polyomavirus Capsid Proteins with Importins Is Required for Efficient Import of Viral DNA into the Cell Nucleus.
Soldatova I, et al.
Viruses, 10(4), 165-165 (2018)
Proximal Ligation Assay (PLA) on Lung Tissue and Cultured Macrophages to Demonstrate Protein-protein Interaction.
Mendez R and Santanu B
Bio-protocol, 7(21) (2017)
Roberto Di Maio et al.
Science translational medicine, 10(451) (2018-07-27)
Missense mutations in leucine-rich repeat kinase 2 (LRRK2) cause familial Parkinson's disease (PD). However, a potential role of wild-type LRRK2 in idiopathic PD (iPD) remains unclear. Here, we developed proximity ligation assays to assess Ser1292 phosphorylation of LRRK2 and, separately
Kan V Lu et al.
Cancer cell, 22(1), 21-35 (2012-07-14)
Inhibition of VEGF signaling leads to a proinvasive phenotype in mouse models of glioblastoma multiforme (GBM) and in a subset of GBM patients treated with bevacizumab. Here, we demonstrate that vascular endothelial growth factor (VEGF) directly and negatively regulates tumor
Afficher tous les articles scientifiques apparentés

Articles

Detection of Protein-protein Interactions by in situ PLA

Proteins are complex biological molecules essential for cellular structure and functions. The majority of proteins commonly interact with various molecules, including other proteins, in order to exert their functions.

How to Optimize the Duolink® Proximity Ligation Assay

Things to consider for preparation, setup and execution of the Duolink® assay protocol

Duolink® References

Find Duolink references based on the type of method used, post translational modification detected, and research focus.

Duolink® PLA Troubleshooting Guide

Support information including tips and tricks, frequently asked questions, and basic troubleshooting.

Protocoles

Duolink® PLA Brightfield Protocol

This protocol describes the use of Duolink® PLA reagents for the brightfield detection, visualization, and quantification of individual proteins, protein modifications, and protein interactions in tissue and cell samples.

Duolink® PLA Flow Cytometry Protocol

Protocol for use of Duolink® PLA reagents for the detection of individual proteins, protein modifications, and protein-protein interactions within cell populations by flow cytometry.

Contenu apparenté

Duolink® PLA Applications

Applications to detect, quantify and visualize protein-protein interactions, post-translational modifications and low expression protein detection using proximity ligation assay

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

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