CSTVRS
shARN MISSION®
Custom Lentiviral Particles
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About This Item
Code UNSPSC :
12352200
Niveau de qualité
Forme
liquid
Technique(s)
capture ELISA: 106 VP/mL using p24
Conditions d'expédition
dry ice
Température de stockage
−70°C
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Catégories apparentées
Description générale
Notre offre complète de produits de type shARN comprend plus de 150 000 constructions de shARN préclonées ciblant plus de 15 000 gènes humains et plus de 15 000 gènes de souris. La banque est classée de plusieurs façons : par groupes de familles géniques (collections de gènes associées à des classes fonctionnelles spécifiques telles que les kinases et les canaux ioniques) ; par groupes de gènes ciblés (groupes de clones de shARN ciblant votre gène de prédilection) ; et par clones de shARN individuels. Nos plateformes de fabrication de shARN et de lentivirus possèdent des laboratoires dédiés, équipés de systèmes de gestion de l'information du laboratoire (LIMS) et de robots de manipulation des liquides à la pointe de la technologie, garantissant les services et la qualité supérieure dont vous avez besoin pour vos activités de recherche sur l'ARNi.
We provide innovative solutions for academic research, biotech research and pharmaceutical drug discovery. Our excellent systems, equipment, and personnel provide the highest quality tools that you require.
Custom Cloning - Now with TurboGFP™
There may be times when classic drug selection for stable or semi-stable cell lines is not possible or desired. In addition, orthogonal methods of selection from puromycin may be required for the expression of multiple shRNAs or expression of shRNA concomitant with transgene expression.
We now offer vectors containing tGFP as a solution to these problems. This vector maintains all of the other elements of the pLKO.1 vector, but the puromycin resistance gene has been replaced by TurboGFP. Our MISSION bio-production team will customize the vector with either a TRC shRNA sequence or your favorite shRNA sequence. With TurboGFP in the vector, FACS and fluorescence microscopy techniques allow for rapid estimation of transduction efficiency and automated, rapid selection of cell populations that stably express the shRNA. Selection by GFP provides an orthogonal method when cells already stably express puromycin resistance. In addition, we also offer pLKO.1-puro-CMV-tGFP. With this vector, you can choose between GFP and puromycin selection, all with the shRNA expression vector.
For selection by a second class of antibiotics, we offer vectors containing the neomycin resistance gene. These two vectors are pLKO.1-hPGK-Neo and pLKO.1-CMV-Neo. Stable shRNA integrants can be found by selecting in G418 (Product Number A1720). As with puromycin, a cytotoxicity profile for your particular cell line will need to be generated. As an example, see the puromycin protocol, but utilize G418 instead of puromycin. As a starting point, 1 mg/mL of G418 has been utilized for selection of shRNA constructs in A549 cells.
The service includes cloning, sequencing, DNA preparation (500 ng) and lentiviral preparation (200 μl at 1 x 106 VP/mL, minimum titer via p24 assay). The DNA and viral preparations are available to be shipped 4-6 weeks from the time the order is placed. For ordering, visit our custom shRNA ordering page or contact our sales representative.
Large-Scale and High Titer Lentiviral Particles
We have a versatile manufacturing platform for high-throughput or large-scale viral production of most HIV-1-based lentiviral vectors. The standard format of the MISSION TRC shRNA collection is 200 μl of 1 x 106 VP/mL (via p24 assay) minimum titer. Any clone, target set, or control can be produced at larger volumes with the same minimum titer specification or higher titers. Our highest titer option is 109 VP/mL. We also accommodate large-scale and high titer viral preparation using clones you send to us (must be compatible with our system). Our robust and optimized processes will save you time and reduce variability in your transduction experiments. To review pricing or to submit a request for a quotation on your specific project, please use our MISSION Custom Request Submission Form.
Custom Cloning - Now with TurboGFP™
There may be times when classic drug selection for stable or semi-stable cell lines is not possible or desired. In addition, orthogonal methods of selection from puromycin may be required for the expression of multiple shRNAs or expression of shRNA concomitant with transgene expression.
We now offer vectors containing tGFP as a solution to these problems. This vector maintains all of the other elements of the pLKO.1 vector, but the puromycin resistance gene has been replaced by TurboGFP. Our MISSION bio-production team will customize the vector with either a TRC shRNA sequence or your favorite shRNA sequence. With TurboGFP in the vector, FACS and fluorescence microscopy techniques allow for rapid estimation of transduction efficiency and automated, rapid selection of cell populations that stably express the shRNA. Selection by GFP provides an orthogonal method when cells already stably express puromycin resistance. In addition, we also offer pLKO.1-puro-CMV-tGFP. With this vector, you can choose between GFP and puromycin selection, all with the shRNA expression vector.
For selection by a second class of antibiotics, we offer vectors containing the neomycin resistance gene. These two vectors are pLKO.1-hPGK-Neo and pLKO.1-CMV-Neo. Stable shRNA integrants can be found by selecting in G418 (Product Number A1720). As with puromycin, a cytotoxicity profile for your particular cell line will need to be generated. As an example, see the puromycin protocol, but utilize G418 instead of puromycin. As a starting point, 1 mg/mL of G418 has been utilized for selection of shRNA constructs in A549 cells.
The service includes cloning, sequencing, DNA preparation (500 ng) and lentiviral preparation (200 μl at 1 x 106 VP/mL, minimum titer via p24 assay). The DNA and viral preparations are available to be shipped 4-6 weeks from the time the order is placed. For ordering, visit our custom shRNA ordering page or contact our sales representative.
Large-Scale and High Titer Lentiviral Particles
We have a versatile manufacturing platform for high-throughput or large-scale viral production of most HIV-1-based lentiviral vectors. The standard format of the MISSION TRC shRNA collection is 200 μl of 1 x 106 VP/mL (via p24 assay) minimum titer. Any clone, target set, or control can be produced at larger volumes with the same minimum titer specification or higher titers. Our highest titer option is 109 VP/mL. We also accommodate large-scale and high titer viral preparation using clones you send to us (must be compatible with our system). Our robust and optimized processes will save you time and reduce variability in your transduction experiments. To review pricing or to submit a request for a quotation on your specific project, please use our MISSION Custom Request Submission Form.
Autres remarques
Pour commander des services de shARN sur mesure, suivez le lien services de shARN sur mesure ou contactez-nous par email à l′adresse MISSIONRNAi@sial.com.
Pour consulter les protocoles et les données relatives aux différentes applications, rendez-vous sur sigma.com/shrna.
Informations légales
L'utilisation de ce produit est soumise à l'octroi d'un ou plusieurs contrats de licence.
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
TurboGFP is a trademark of Evrogen Co.
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Articles
MISSION shRNA reduce the expression of specific target genes by targeting the specific mRNA therefore reducing the corresponding protein expression.
Contenu apparenté
Find shRNAs for Individual Genes. Over 150,000 pre-cloned shRNA constructs targeting more than 15,000 human and 15,000 mouse genes.
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
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