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CS0780

Sigma-Aldrich

β-N-Acetylglucosaminidase Assay Kit

sufficient for 50 reactions (1 mL), sufficient for 500 reactions (100 μL)

Synonyme(s) :

Beta-NAG Activity Assay Kit

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About This Item

Numéro CE :
222-398-7
Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84

Utilisation

sufficient for 50 reactions (1 mL)
 sufficient for 500 reactions (100 μL)

Niveau de qualité

200

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Informations sur le gène

human ... NAGLU(4669)

Application

The kit provides the reagents required for a fast and convenient detection of β-N-Acetylglucosaminidase activity in cell lysates, tissue homogenates or purified enzyme. It is a useful tool for the detection of lysosomes in fractionated cell/tissue samples.

Actions biochimiques/physiologiques

N-acetyl-D-glucosaminidase (NAG) in mammals is a lysosomal enzyme, which takes part in the intracellular degradation of glycolipids and glycoproteins. High activities of this enzyme have been detected in human kidney, lung and liver lysosomes. Elevated levels of serum NAG are associated with certain forms of cancer.

Remarque sur l'analyse

The kit assay is based on the hydrolysis of NAG substrate (NP-GLcNAc) by the enzyme. The enzymatic hydrolysis of the substrate liberates p-nitrophenylate ion. The reaction product is detected colorimetrically at 405 nm.

Autres remarques

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Composants de kit seuls

Réf. du produit
Description
  • Dilution Buffer 8 mL
  • 4-Nitrophenyl-N-acetyl-β-D-glucosaminide 50 mg
  • Citrate Buffer Solution, 0.09 M 100 mL
  • p-Nitrophenol Standard Solution, 10 mM 1 mL
  • β-N-Acetylglucosaminidase from Jack beans 1 vial
  • Sodium carbonate 5 g

Pictogrammes

Health hazardExclamation mark
GHS08,GHS07

Mention d'avertissement

Warning

Mentions de danger

H319,H351,H373

Classification des risques

Carc. 2 - Eye Irrit. 2 - STOT RE 2 Oral

Organes cibles

Liver,Kidney

Code de la classe de stockage

11 - Combustible Solids

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Richard A Zager et al.
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, 28(6), 1455-1462 (2013-04-02)
Recent experimental work suggests a paradox: although uremia evokes systemic toxicities, in the setting of AKI, it can induce intrarenal cytoprotective and anti-inflammatory effects. Whether these influences can attenuate post-ischemic kidney disease progression remains unknown. To explore this possibility, male
Shanshan Wang et al.
Biochemical and biophysical research communications, 483(1), 45-51 (2017-01-09)
Activation of endothelial cells plays a key role in septic acute kidney injury (AKI). This study investigated the role of miRNA in endothelial-induced tubular cell injury in sepsis. Circulating endothelial cells (CECs) from septic AKI, non-septic AKI, septic non-AKI patients
Xiaojia Liu et al.
Acta pharmaceutica Sinica. B, 11(10), 3134-3149 (2021-11-09)
Programmed cell death ligand 1 (PD-L1)/programmed cell death protein 1 (PD-1) cascade is an effective therapeutic target for immune checkpoint blockade (ICB) therapy. Targeting PD-L1/PD-1 axis by small-molecule drug is an attractive approach to enhance antitumor immunity. Using flow cytometry-based
Miguel A Lanaspa et al.
Journal of the American Society of Nephrology : JASN, 25(11), 2526-2538 (2014-05-31)
Diabetes is associated with activation of the polyol pathway, in which glucose is converted to sorbitol by aldose reductase. Previous studies focused on the role of sorbitol in mediating diabetic complications. However, in the proximal tubule, sorbitol can be converted
Xiaoting Chen et al.
Journal of cellular and molecular medicine, 23(6), 4290-4300 (2019-04-12)
Impaired autophagic degradation of intracellular lipids is causally linked to the development of non-alcoholic steatohepatitis (NASH). Pharmacological agents that can restore hepatic autophagic flux could therefore have therapeutic potentials for this increasingly prevalent disease. Herein, we investigated the effects of
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Organelle Isolation

The isolation of subcellular fractions by centrifugation is a commonly used technique and is widely applicable across multiple cell and tissue types. Because organelles differ in their size, shape, and density, centrifugation can be easily employed to separate and purify organelle fractions from gently homogenized samples.

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