10875406001

N-Acetyl-β-D-Glucosaminidase (NAG)

sufficient for ~50 tests

• Synonyme(s) : NAG
• Code UNSPSC : 12352204

Propriétés

• Forme: solid
• Niveau de qualité: 100
• Utilisation: sufficient for ~50 tests
• Poids mol.: 105.99 g/mol
• Fabricant/nom de marque: Roche
• Technique(s): activity assay: suitable
• Couleur: white to light yellow-brown
• Solubilité: water: soluble ~210 g/L at 68 °F (Stop reagent); soluble ~71 g/L at 32 °F (stop reagent); water: soluble (Buffer substance, lyophilized substrate)
• Adéquation: suitable for enzyme test
• Application(s): life science and biopharma
• Température de stockage: room temp

Description

Description générale

N-acetyl-β-D-glucosaminidase (NAG) is mainly used in the colorimetric assay for the determination of N-acetyl-β-D-glucosaminidase in urine. NAG hydrolyzes 3-cresolsulfonphthaleinyl-N-acetyl-D-glucosaminide to release 3-cresolsulfonphthalein, which is measured photometrically at 580nm. It is an intracellular lysosomal enzyme, present in the S3 segment of proximal tubular cells and distal nephron. Presence of NAG in the urine shows an organelle impairment in proximal tubule. Level of NAG rises in individuals over 70 years of age.

Application

N-acetyl-β-D-glucosaminidase (NAG) has been used for preparing standards for the determination of N-acetyl-β-D-glucosaminidase in urine in life-science research applications.

Conditionnement

1 kit containing 3 components

Notes préparatoires

Working solution: Preparation of solutions:

1. Buffer solution
   Dissolve the contents of bottle 1 with 55 ml double-distilledwater.
2. Substrate solution
   Dissolve the contents of bottle 2 with 55 ml solution I.
3. Stop reagent
   Dissolve the contents of bottle 3 with 110 ml double-distilled water.

Storage conditions (working solution): Stability of solutions:
Solution II is stable for 1 month when stored at 2 to 8 °C, protected from light.
Solution III is stable for 1 month stored at 2 to 8 °C.

Stability of the sample:
The activity determination of the N-acetyl-β-D-glucosaminidase (NAG) should be carried out directly after collecting the sample. Turbid urines should be centrifuged and the supernatant decanted. NAG is stable for one week at 2 to 8 °C and for one month when stored at -15 to -25 °C.

Stockage et stabilité

Keep container tightly closed in a dry and well-ventilated place.

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Composants de kit seuls

• Buffer Substance consisting of citric acid and potassium citrate
• Lyophilized Substrate consisting of sodium 3-cresolsulfonphthaleinyl-N-acetyl-β-D-glucosaminide and borax
• Stop Reagent consisting of sodium carbonate

Informations sur la sécurité

• Pictogrammes: GHS07,GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H319,H335,H360FD
• Conseils de prudence: P201 - P261 - P280 - P304 + P340 + P312 - P308 + P313 - P337 + P313
• Classification des risques: Eye Irrit. 2 - Repr. 1B - STOT SE 3
• Organes cibles: Respiratory system
• Code de la classe de stockage: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): does not flash
• Point d'éclair (°C): does not flash