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CRISPR12

Sigma-Aldrich

CRISPR-Lenti Non-Targeting Control Plasmid

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About This Item

Code UNSPSC :
41106609
Nomenclature NACRES :
NA.51

Forme

liquid

Niveau de qualité

100

Conditionnement

vial of 50 μL

Concentration

20 ng/μL in TE buffer; DNA (1μg of plasmid DNA)

Application(s)

CRISPR

Conditions d'expédition

dry ice

Température de stockage

−20°C

Catégories apparentées

Description générale

The Sigma lenti CRISPR Non-Targeting Control is a lentiviral plasmid vector, which includes a gRNA sequence that does not target known human, mouse and rat genes. This vector is useful as a negative control in experiments using Sigma CRISPR lentiviral clones. The Sigma lenti CRISPR non-targeting control uses a one-plasmid system consisting of a EF1-alpha-driven Cas9, a U6-driven guide RNA (non-targeting), with both Puromycin and GFP co-expressed with Cas9.

Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells, respectively. Also, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids (Product No. SHP001).

Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas), originally found in bacteria and archaea, is employed by organisms to protect them from invading viruses and plasmids.

Application

Functional Genomics/Screening/Target Validation
  • Non-targeting control, CRISPR lentivirus DNA format, for creating gene knockouts/genetic modifications in cell lines.

To see more application data, protocols, vector maps visit sigma.com/crisprs.

Caractéristiques et avantages

Serves as a non-targeting or negative experimental control (DNA format) for the CRISPR editing workflow using lenti CRISPRs. This allows you to examine the effect of transfection in your system with the Sigma lenti CRISPR/Cas9 plasmid.

Forme physique

The Sigma CRISPR Lenti Non-Targeting Control Vector is provided as 1 μg of plasmid DNA in Tris-EDTA (TE).

Informations légales

CRISPR Use License Agreement

Lentiviral and WPRE License Agreements

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
02052116MN
06111913MN

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

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Protocoles

CRISPR Cas 9 Nuclease RNA-guided Genome Editing

Learn about CRISPR Cas9, what it is and how it works. CRISPR is a new, affordable genome editing tool enabling access to genome editing for all.

Contenu apparenté

Visualize your CRISPR Experiment with MISSION

Watch Cas9-GFP proteins work inside your cells and deliver the powerful nuclease editing you have come to trust and expect. MISSION™ SpCas9-GFP and eSpCas9-GFP proteins are multi-functional enzymes with high on-target cleavage efficiency fused to Enhanced Green Fluorescent Protein (EGFP) for real-time observation of Cas9 delivery and clearance from cells.

Global Trade Item Number

RéférenceGTIN
CRISPR12-1EA4061833541289
CRISPR12N-1EA4065269393337

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