Jurkat E6.1 Cell Line human

from human blood(leukemic T-cell lymphoblast), 88042803

• Synonyme(s) : Jurkat Cells, Jurkat Clone E6-1 Cells
• Code UNSPSC : 41106514

Propriétés

• Source biologique: human blood (leukemic T-cell lymphoblast)
• Mode de croissance: Suspension
• Caryotype: Pseudodiploid, modal No 46
• Morphologie: Not specified
• Produits: Interleukin 2 (IL-2), human alpha interferon
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human leukaemic T cell lymphoblast

Description de la lignée cellulaire

Derived from Jurkat FHCRC. An IL-2 producing cell line, derived by incubating the cells at 41°C for 48 hours followed by a limiting dilution cloning over macrophages.

Application

IL-2 assay and cloning studies

Jurkat E6.1 cell line has been used to:

• study cell-independent/vector-free delivery of bovine serum albumin-fluorescein conjugate
• determine the cytotoxicity of the cytolethal distending toxin (CDT) holotoxin and its components
• study the expression of bisphenol A exposed estrogen receptor-β (ERβ) and estrogen-related-receptor-α (ERRα) in vitro

Profil d'ADN

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 8,12
D16S539: 11
D5S818: 9
D7S820: 8,10
THO1: 6,9.3
TPOX: 8,10
vWA: 18

Milieu de culture

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. On resuscitation single cells can be observed, during culture most cells will grow as aggregates.

Autres remarques

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