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Merck
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SLM-240

Sigma-Aldrich

RPMI-1640 complete medium

With ʟ-glutamine and 10% US origin FBS, liquid, sterile-filtered, suitable for cell cutlure

Synonyme(s) :

Low Bicarb Formulation, Roswell Park Memorial Institute 1640 medium

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About This Item

Code UNSPSC :
12352207
eCl@ss :
32160801
Nomenclature NACRES :
NA.71

product name

RPMI 1640 Complete Medium, with 2mM L-Glut and 10% FBS, supplemented with 2mM L-Glutamine and 10% U.S. origin FBS

Niveau de qualité

300

Forme

liquid

Technique(s)

cell culture | mammalian: suitable

Description générale

RPMI 1640 CompleteMedium is supplemented with 2mM L-Glutamine and 10% U.S.-origin fetal bovineserum (FBS). This sterile solution is convenient to culture a wide range ofcells that grow in suspension or as anchorage dependent. It can also be used infusion protocols and for growing hybrid cells. Roswell Park Memorial Institute(RPMI) 1640 medium can be used for culturing human normal and neoplasticleukocytes. It supports the growth of various cultured cells, like fresh humanlymphocytes in the 72-hour phytohemagglutinin (PHA) stimulation assay if theformulation is properly supplemented.

Application

RPMI 1640 CompleteMedium, with 2mM L-Glut and 10% FBS has been used to culture: Jurkatlymphocytes and human U937 monocyte-like cell line(1), HT-29 and SW480 (humancolon cancer cell line) cells(2), monocytes(3)

Qualité

pH= 7.2-7.5
Osmolality= 270-292 mOsm/kg
Endotoxin= ≤2 EU/mL
Mycoplasma= Negative
Sterility Tested= Negative for Growth
Appearance= Orange/Red Clear Liquid
Cell Line Tested= A2780 Human Ovarian Carcinoma

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Yimei Jiang et al.
BMC molecular and cell biology, 21(1), 48-48 (2020-07-01)
Colorectal cancer (CRC) is a leading cancer and a major cause of death. Lipopolysaccharide (LPS), an abundant component in gut microbiome, is involved in CRC progression and metastasis, potentially through regulating the miRNA composition of CRC-derived exosomes. In this study
Anca Ungurianu et al.
Oxidative medicine and cellular longevity, 2020, 3580934-3580934 (2020-07-21)
Ionizing radiation induces genomic instability in living organisms, and several studies reported an ageing-dependent radiosensitivity. Chemical compounds, such as scavengers, radioprotectors, and modifiers, contribute to reducing the radiation-associated toxicity. These compounds are often antioxidants, and therefore, in order to be
Cristina C Clement et al.
Nature communications, 12(1), 4447-4447 (2021-07-23)
Tryptophan catabolism is a major metabolic pathway utilized by several professional and non-professional antigen presenting cells to maintain immunological tolerance. Here we report that 3-hydroxy-L-kynurenamine (3-HKA) is a biogenic amine produced via an alternative pathway of tryptophan metabolism. In vitro

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