C3735
Cytochrome P450 human
1A1 Isozyme Microsomes, with P450 Reductase, recombinant, expressed in baculovirus infected insect cells (BTI-TN-5B1-4)
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About This Item
Numéro MDL:
Code UNSPSC :
12161501
Nomenclature NACRES :
NA.47
Produits recommandés
Source biologique
human
Niveau de qualité
Produit recombinant
expressed in baculovirus infected insect cells (BTI-TN-5B1-4)
Forme
solution
Activité spécifique
≥4 units/pmol enzyme
Poids mol.
45-60 kDa
Conditionnement
vial of 0.5 nmol
Technique(s)
activity assay: suitable
Solubilité
water: soluble
Adéquation
suitable for molecular biology
Numéro d'accès UniProt
Application(s)
cell analysis
Conditions d'expédition
dry ice
Température de stockage
−70°C
Informations sur le gène
human ... CYP1A1(1543)
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Description générale
Research area: Immuno and CKS
Cytochrome P450 (CYP) enzymes are a family of enzymes that are encoded by the P450 genes. These enzymes are membrane bound hemoproteins. that are mainly found in the liver’s endoplasmic reticulum.
Cytochrome P450 (CYP) enzymes are a family of enzymes that are encoded by the P450 genes. These enzymes are membrane bound hemoproteins. that are mainly found in the liver’s endoplasmic reticulum.
Actions biochimiques/physiologiques
Cytochrome P450 is a heterogeneous family of isozymes whose primary function is to oxidize small molecules, both as a function of intermediary metabolism (e.g., fatty acids) and to detoxify exogenous compounds (drugs or toxins). Some isoforms have narrow substrate specificity, while others are promiscuous. The CYP1A1 isoform catalyzes 7-deethylation of ethoxyresorufin. Cytochrome P450 (CYP) plays an important role in detoxifying xenobiotics, cellular metabolism and homeostasis. One of the main mechanisms of drug-drug interactions is the induction or inhibition of these enzymes. CYP enzymes are transcriptionally activated by a variety of xenobiotics and by endogenous substrates via receptor-dependent pathways. Inhibition of these enzymes is a major factor in metabolism-based drug-drug interactions, and many chemotherapeutic medications can cause drug interactions by either inhibiting or inducing the cytochrome p450 enzyme system.
Forme physique
Solution in 100 mM potassium phosphate buffer, pH 7.4.
Notes préparatoires
Microsomes containing human CYP1A1 and recombinant human NADPH-P450 reductase.
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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Les clients ont également consulté
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Toxicology in vitro : an international journal published in association with BIBRA, 59, 215-220 (2019-04-21)
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PloS one, 9(4), e94962-e94962 (2014-04-17)
The present study characterized in vitro metabolites of 20(R)-25-methoxyl-dammarane-3β, 12β, 20-triol (20(R)-25-OCH3-PPD) in mouse, rat, dog, monkey and human liver microsomes. 20(R)-25-OCH3-PPD was incubated with liver microsomes in the presence of NADPH. The reaction mixtures and the metabolites were identified
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