B0939

BAFF active human

Animal-component free, recombinant, expressed in Nicotiana, >97% (SDS-PAGE)

• Synonyme(s) : B lymphocyte stimulator, B-cell-activating factor BAFF, BLyS, Dendritic cell-derived TNF-like molecule, TALL-1, TNF- and APOL-related leukocyte expressed ligand 1
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human
• Niveau de qualité: 200
• Produit recombinant: expressed in Nicotiana
• Pureté: >97% (SDS-PAGE)
• Forme: lyophilized
• Puissance: ≤50 ng/mL ED₅₀
• Poids mol.: 18-20 kDa (a glycosilated polypeptide chain containing 151 amino acids)
• Conditionnement: pkg of 10 μg
• Conditions de stockage: avoid repeated freeze/thaw cycles
• Impuretés: Endotoxin, tested (LAL test, < 0.04 EU/ ug protein)
• Numéro d'accès UniProt: Q9Y275
• Température de stockage: −20°C
• Informations sur le gène: human ... TNFSF13B(10673)

Description

Description générale

BAFF (B lymphocyte activating factor) is a member of the tumor necrosis factor (TNF) ligand family which is expressed in T Cells, macrophages, monocytes and dendritic cells. It is also known as BLyS, THANK, TALL, zTNF4 and TNFS20. BAFF enhances B cell survival in vitro and has emerged as a key regulator of peripheric B cell and it is vital homeostatic cytokine for B cells that helps regulate both innate and adaptive immune responses. BAFF binds to three TNF receptors: B-cell maturation antigen (BCMA/TNFRSF17), transmembrane activator and calcium modulator and cyclophilin ligand interactor(TACI/TNFRSF13B) and BAFF receptor (BAFF R/BR3/TNFRSF 13C).The human BAFF gene code for a 285 amino acids type II transmembrane protein. Recombinant human soluble BAFF is a 151 amino acids containing the TNF-like portion of the extracellular domain of BAFF.

Forme physique

Lyophilized from a 20 mM PBS buffer at pH 7 and 0.2 M NaCl.

Reconstitution

Lyophilized protein should be reconstituted in water to a concentration of 25 - 50 ng /μl. Due to the protein nature, dimmers and multimers may be observed.

Remarque sur l'analyse

The activity is determined by dose-dependeant stimulation of proliferation B cell from Human PBMC. Cell proliferation was measured by MTT method.
*activity results may vary with PBMC donors.
ED₅₀ ≤ 50 ng/mL

Autres remarques

Extinction Coefficient: E^0.1% = 0.791 (A₂₈₀ nm)
p.I: 6.03

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable