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83330

Sigma-Aldrich

Pyruvate Kinase from rabbit muscle

crystalline suspension in 3.8 M ammonium sulfate solution, ~350 U/mg protein (10-40 mg/ml)

Synonyme(s) :

ATP:pyruvate 2-O-phosphotransferase, PK

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About This Item

Numéro CAS:
9001-59-6
Numéro de classification (Commission des enzymes):
2.7.1.40 (BRENDA, IUBMB)
Numéro CE :
232-616-2
Numéro MDL:
MFCD00081946
Code UNSPSC :
12352204
eCl@ss :
32160410
Nomenclature NACRES :
NA.54

Forme

suspension

Qualité

crystalline suspension in 3.8 M ammonium sulfate solution

Activité spécifique

~350 U/mg protein (10-40 mg/ml)

Poids mol.

Mr ~230000

pH

~6

Activité étrangère

lactate dehydrogenase ≤0.01%

Température de stockage

2-8°C

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Actions biochimiques/physiologiques

Molecular Weight: 237 kDa and exists as a tetramer of four equal subunits of molecular weight 57 kDa.
Isoelectric Point: 7.6
Optimal pH: ∼7.5
Optimal Temperature: 25°C
ΕA280 = 0.54 for 1 mg(p)/ml, 1 cm path
Reported KM values are ATP (0.86 mM), pyruvate (10 mM), ADP (0.3 mM), and PEP (0.07 mM) in Tris buffer at pH 7.4 and 30 °C. Pyruvate kinase is highly specific for phosphoenolpyruvate, but can utilize other dinucleotide triphosphates as substrates in place of ATP including GTP, ITP, dATP, UTP, and CTP.
Pyruvate kinase catalyzes the conversion of phosphoenolpyruvate to pyruvate, whitch is the rate-limiting final step in glycolysis.

Définition de l'unité

1 U corresponds to the amount of enzyme which catalyzes the transphosphorylation of 1 μmol PEP per min at pH 7.4 and 25 °C

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Nicolas Galazis et al.
European journal of endocrinology, 168(2), R33-R43 (2012-10-25)
Women with polycystic ovary syndrome (PCOS) are at increased risk of developing insulin resistance and type 2 diabetes mellitus (T2DM). In this study, we attempted to list the proteomic biomarkers of PCOS and T2DM that have been published in the
William J Cook et al.
PloS one, 7(10), e46875-e46875 (2012-10-12)
Pyruvate kinase plays a critical role in cellular metabolism of glucose by serving as a major regulator of glycolysis. This tetrameric enzyme is allosterically regulated by different effector molecules, mainly phosphosugars. In response to binding of effector molecules and substrates
Daniel I Benjamin et al.
Cell metabolism, 16(5), 565-577 (2012-10-16)
Cancer cells possess fundamentally altered metabolism that provides a foundation to support tumorigenicity and malignancy. Our understanding of the biochemical underpinnings of cancer has benefited from the integrated utilization of large-scale profiling platforms (e.g., genomics, proteomics, and metabolomics), which, together
Mayumi Tamada et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 18(20), 5554-5561 (2012-10-17)
The M2 splice isoform of pyruvate kinase (PKM2), an enzyme that catalyzes the later step of glycolysis, is a key regulator of aerobic glycolysis (known as the Warburg effect) in cancer cells. Expression and low enzymatic activity of PKM2 confer
Jidong Cheng et al.
Genes to cells : devoted to molecular & cellular mechanisms, 17(11), 913-922 (2012-10-20)
AMP deaminase (AMPD) catalyzes AMP to IMP and plays an important role in energy charge and nucleotide metabolism. Human AMPD3 deficiency is a type of erythrocyte-specific enzyme deficiency found in individuals without clinical symptoms, although an increased level of ATP
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