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30326

Sigma-Aldrich

EMPIGEN® BB detergent

~30% active substance

Synonyme(s) :

N,N-Dimethyl-N-dodecylglycine betaine, N-(Alkyl C10-C16)-N,N-dimethylglycine betaine

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About This Item

Formule linéaire :
CH3(CH2)8-14CH2N+(CH3)2CH2COO-
Numéro CAS:
66455-29-6
Numéro Beilstein :
3670807
Numéro CE :
266-368-1
Numéro MDL:
MFCD00084742
Code UNSPSC :
12161900
ID de substance PubChem :
329753464
Nomenclature NACRES :
NA.25

Description

zwitterionic

Niveau de qualité

200

Forme

liquid

Composition

solid content, 35.0-45.0 % (w/v)

Concentration

~30% active substance

CMC

1.6-2.1 mM (20-25°C)

Densité

1.030-1.060 g/mL at 20 °C

Absorption UV

λ: 417 nm Amax: ≤0.055
λ: 441 nm Amax: ≤0.037

Chaîne SMILES 

CCCCCCCCCCCC[N+](C)(C)CC([O-])=O

InChI

1S/C16H33NO2/c1-4-5-6-7-8-9-10-11-12-13-14-17(2,3)15-16(18)19/h4-15H2,1-3H3

Clé InChI

DVEKCXOJTLDBFE-UHFFFAOYSA-N

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Catégories apparentées

Application

Empigen BB is a zwitterionic detergent compatible with anionic, non-ionic, cationic and other amphoteric surfactants.

Empigen BB is a useful detergent that has been used in the solubilization of membrane proteins and biochemical analysis of keratins. It not only helps to solubilize bigger amounts of protein but it also maintains antibody reactivity. It has been also used in the purification of leucine-rich repeat kinase 2 (LRRK2) in Escherichia coli during research in Parkinson′s disease. The use of Empigen in the preparation of viral antigens is being increasing due to its effectivity in giving greater antibody responses.

Informations légales

EMPIGEN is a registered trademark of Huntsman Corporation

Pictogrammes

Exclamation mark
GHS07

Mention d'avertissement

Warning

Mentions de danger

H315,H319,H335

Classification des risques

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Organes cibles

Respiratory system

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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Islam Khan et al.
Microbes and infection, 7(5-6), 853-859 (2005-05-10)
Campylobacter jejuni is a major food-borne pathogen and a leading cause of diarrhoea. A cytotoxin is most likely involved in the pathogenesis of inflammatory diarrhoea due to C. jejuni. A 45-kDa outer membrane protein encoded by the porA gene was
Kulbir Singh et al.
Langmuir : the ACS journal of surfaces and colloids, 30(13), 3673-3680 (2014-03-15)
In this article, we report additive-induced micellar growth and rheology modification for mixtures of anionic (sodium dodecyl sulfate, SDS) and zwitterionic (N-alkylated glycine derivative, Empigen BB or EBB) surfactants. Two nonaromatic hydrotropic salts (hexyltrimethylammonium bromide, C6TAB, and/or dibutylenebis(dimethylbutylammonium bromide), 4-4-4)
L A Lowthert et al.
Biochemical and biophysical research communications, 206(1), 370-379 (1995-01-05)
Intermediate filament (IF) proteins make up some of the most insoluble proteins known, and within the IF protein family, keratins are the least soluble. We compared the efficiency of nonionic, cationic, mixed nonionic and anionic, and zwitterionic detergents in solubilizing
Y Rinke et al.
Virology, 170(2), 424-432 (1989-06-01)
We have established a sensitive assay for the quantitative determination of large T antigen determinants on the surface of living simian virus 40 (SV40)-transformed cells (mKSA). Cells in suspension culture were incubated with monoclonal antibodies specific for large T antigen
Wenjin Guo et al.
Protein expression and purification, 90(1), 34-39 (2013-05-15)
Vaccinia virus (VV) has been used to express a variety of heterologous proteins, including HIV envelope (Env) glycoproteins. The Env protein is synthesized as a precursor molecule, gp160, which is cleaved into the surface antigen gp120 and the transmembrane protein
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