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12306C

Sigma-Aldrich

Sérum de veau fœtal

non-USA origin, from USDA approved countries, Heat Inactivated, sterile-filtered, suitable for cell culture

Synonyme(s) :

FBS, FCS, sérum, sérums

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About This Item

Numéro MDL:
MFCD00132239
Code UNSPSC :
12352207
Nomenclature NACRES :
NA.71

Source biologique

bovine fetus

Stérilité

sterile-filtered

Gamme de produits

FBS Premier

Qualité

from USDA approved countries

Composition

Hemoglobin, ≤25 mg/dL

Origine

non-USA origin

Technique(s)

cell culture | mammalian: suitable

Impuretés

≤10 EU/mL endotoxin

Température de stockage

−20°C

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Catégories apparentées

Application

FBS sourced from USDA approved non-USA countries is used in a broad range of cell culture applications. FBS provides many non-defined growth promoting and survival enhancing factor to cells in culture.
Fetal Bovine Serum has been used to block the human induced pluripotent stem cells (hiPSCs) derived hepatocyte-like cells (HLCs) cells in phosphate buffered saline during immunofluorescence staining.

Actions biochimiques/physiologiques

Fetal Bovine Serum (FBS) is used as a supplement to cell culture media. It provides many non-defined growth promoting and survival enhancing factors to cells in culture.

Adéquation

USDA Approved Sourcing refers to FBS produced from blood collected in countries outside of the United States that have been approved by the USDA (United States Department of Agriculture) for export of beef products into the United States. Eligible countries for export into the U.S. include Australia, Canada, Chile, Costa Rica, Honduras, Iceland, Japan, Mexico, New Zealand, Nicaragua, Uruguay. Foreign serum is tested to ensure the absence of exotic viruses before release by the USDA.

Remarque sur l'analyse

  • Taux d′endotoxines et d′hémoglobine testés
  • Tested for the presence of bacteria, virus, and mycoplasma
  • Triple filtered with 0.1 micron membrane under aseptic conditions

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves

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Certificats d'analyse (COA)

Lot/Batch Number
17L186
14E314H1
13G114H1
12C483H1

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Sérum de veau fœtal USA origin, γ-irradiated by SER-TAIN process, sterile-filtered, suitable for cell culture

Sigma-Aldrich

12107C

Sérum de veau fœtal

Eppendorf® ep Dualfilter T.I.P.S.® PCR clean, sterile, tip volume × L 50-1250 μL × 103 mm, dark green tip, case of 480 tips (5 racks x 96 tips)

EP0030078594

Eppendorf® ep Dualfilter T.I.P.S.®

Differentiation and transplantation of human induced pluripotent stem cell-derived hepatocyte-like cells
Asgari S, et al.
Stem Cell Reviews and Reports, 9(4), 493-504 (2013)
Generation of functional hepatocyte-like cells from human pluripotent stem cells in a scalable suspension culture
Vosough M, et al.
Stem Cells and Development, 22(20), 2693-2705 (2013)
Gerhard Gstraunthaler
ALTEX, 20(4), 275-281 (2003-12-13)
Serum is commonly used as a supplement to cell culture media. It provides a broad spectrum of macromolecules, carrier proteins for lipoid substances and trace elements, attachment and spreading factors, low molecular weight nutrients, and hormones and growth factors. The
Xiao-Min Liu et al.
Nature chemical biology, 15(9), 865-871 (2019-08-07)
RNA modification in the form of N6-methyladenosine (m6A) regulates nearly all the post-transcriptional processes. The asymmetric m6A deposition suggests that regional methylation may have distinct functional consequences. However, current RNA biology tools do not distinguish the contribution of individual m6A
Michael P Collins et al.
The Journal of biological chemistry, 295(28), 9433-9444 (2020-05-16)
The vacuolar H+-ATPase (V-ATPase) is an ATP-dependent proton pump that is essential for cellular homeostasis. V-ATPase activity is controlled by the regulated assembly of the enzyme from its component V1 and V0 domains. We previously reported that amino acid starvation
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Cultured cells are excellent hosts for the propagation of many types of viruses. The ability of cell culture systems to produce large quantities of attenuated viral particles has served as the basis for the production of both human and veterinary vaccines. Traditional methods have relied on the production of viral agents in cells cultured in medium supplemented with serum, most commonly fetal bovine serum (FBS).

Fetal Bovine Serum (FBS) – What You Need to Know

Review the basics of using fetal bovine (or calf) serum for cell culture and how sourcing and regulations affect the price of sera for cell-based assays.

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Discover our comprehensive portfolio of fetal bovine sera (FBS) and determine the right FBS for your lab needs and budget. Learn about how global events affect FBS pricing.

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