Accéder au contenu
Merck
Toutes les photos(1)

Principaux documents

Voir toute la documentation

ATG004

Sigma-Aldrich

A375 NY−ESO−1 Antigen Panel Cell Lines

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(1)

About This Item

Code UNSPSC :
41106514
Nomenclature NACRES :
NA.41

Source biologique

human skin (malignant melanoma)

Mode de croissance

adherent

Morphologie

epithelial

Conditions d'expédition

dry ice

Température de stockage

−196°C

Description générale

This product is a panel of three (3) genetically modified cell lines involving the NY-ESO-1 gene. The panel consists of a NY-ESO-1 knockout cell line (NY-ESO-1-/-) as well as a NY-ESO-1low and a NY-ESO-1high expression cell line. The NY-ESO-1-/- cell line was generated using CompoZr® Zinc Finger Nuclease technology, and the two NY-ESO-1 expression cell lines were generated using MISSION® lentivirus. The NY-ESO-1low and NY-ESO-1high cell lines express NY-ESO-1 at low and high levels relative to the NY-ESO-1-/- cell line (NY-ESO-1high > NY-ESO-1low > NY-ESO-1-/-) as measured via fluorescence-activated cell sorting (FACS). As a comparison, wild type A375 cells demonstrate NY-ESO-1medium expression as measured via FACS.

The STR profile of these cell lines match that of its parental cell line - ECACC catalog number 88113005.

Description de la lignée cellulaire

Derived from a 54 year old female with malignant melanoma. The cell line produces rapidly growing amelanotic melanomas in anti-thymocyte serum treated NIH Swiss mice.

Caractéristiques et avantages

The A375 NY-ESO-1 antigen panel cell lines feature NY-ESO-1 expressed at varying levels (KO, low, and high). These cell lines enable researchers to screen and evaluate the efficacy and potency of NY-ESO-1-targeted immunotherapies in a biologically-relevant cellular research model.

Composants

ATG004A-1VL: A375 NY-ESO-1 Knockout
ATG004B-1VL: A375 NY-ESO-1 Low
ATG004C-1VL: A375 NY-ESO-1 High

Qualité

Tested for Mycoplasma, bacterial and fungal content, post-freeze viability, and short terminal repeat (STR) analysis for cell line identification.

Informations légales

MISSION Use Licenses Agreement

ZFN Use Licenses Agreement
CompoZr is a registered trademark of Merck KGaA, Darmstadt, Germany
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

Code de la classe de stockage

10 - Combustible liquids

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number

Désolés, nous n'avons pas de COA pour ce produit disponible en ligne pour le moment.

Si vous avez besoin d'assistance, veuillez contacter Service Clients

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Articles

Immuno Oncology Research Tools and Solutions

Whether you need screening tools for CAR/TCR therapies and mAB development, target cells for efficacy testing, or potency assays for clinical development, we provide functional biologically-relevant solutions to help you save time, accelerate your immuno-oncology research, and simplify your workflow.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique