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66721

Sigma-Aldrich

Methylene Blue zinc chloride double salt

for microscopy (Bact., Bot., Hist.), ~1 mol/mol water

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About This Item

Formule empirique (notation de Hill):
C16H18ClN3S · 0.5ZnCl2 · xH2O
Numéro CAS:
26283-09-0
Poids moléculaire :
388.00 (anhydrous basis)
Numéro Beilstein :
3839953
Numéro MDL:
MFCD00150006
Code UNSPSC :
12352106
ID de substance PubChem :
329760311
Nomenclature NACRES :
NA.25

Qualité

for microscopy (Bact., Bot., Hist.)

Niveau de qualité

100

Impuretés

~1 mol/mol water

Chaîne SMILES 

O.[Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C

InChI

1S/C16H18N3S.ClH.H2O/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;;/h5-10H,1-4H3;1H;1H2/q+1;;/p-1

Clé InChI

WQVSELLRAGBDLX-UHFFFAOYSA-M

Application

Methylene blue is a heterocyclic aromatic chemical compound used as a cationic dye. It is used as a redox indication in analytical chemistry. Methylene blue is a component of a number of staining protocols such as Wright′s stain and Jenner′s stain for microscopic evaluation.

Pictogrammes

Skull and crossbones
GHS06

Mention d'avertissement

Danger

Mentions de danger

H301,H319

Classification des risques

Acute Tox. 3 Oral - Eye Irrit. 2

Code de la classe de stockage

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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Certificats d'analyse (COA)

Lot/Batch Number
404519/1
404519/1
268544/1
376334/1
373545/1

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K P Krafts et al.
Biotechnic & histochemistry : official publication of the Biological Stain Commission, 86(1), 7-35 (2011-01-18)
The components of the blood stain, eosin and methylene blue, were introduced by Baeyer and Caro, respectively. Methylene blue was used primarily for detecting Mycobacterium tuberculosis until Ehrlich in 1880 mixed methylene blue with acid fuchsin to produce what he
A Van Deun et al.
The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease, 14(12), 1571-1575 (2010-12-15)
Damien Foundation Bangladesh tuberculosis (TB) control projects. To compare blue ink, potassium permanganate and methylene blue background staining for transmitted light-emitting diode (LED) TB fluorescence microscopy (FM). Auramine smears made in triplicate from Ziehl-Neelsen (ZN) acid-fast bacilli (AFB) positive or
Kristine Krafts et al.
Parasitology research, 109(3), 521-529 (2011-06-11)
Methylene blue was synthesized by Caro in 1876 at BASF, a chemical company. Six years later, Koch employed methylene blue when he discovered the tubercle bacillus. In 1880, Ehrlich described what he termed "neutral" dyes: mixtures of acidic and basic

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