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11669915001

Roche

Cell Proliferation ELISA, BrdU (chemiluminescent)

Synonyme(s) :

BrdU, cell proliferation

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About This Item

Code UNSPSC :
41116158

Utilisation

sufficient for ≤1,000 tests

Niveau de qualité

100

Fabricant/nom de marque

Roche

Technique(s)

ELISA: suitable

Méthode de détection

chemiluminescent

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Catégories apparentées

Description générale

Chemiluminescent immunoassay for the quantification of cell proliferation, based on the measurement of BrdU incorporation during DNA synthesis:  a nonradioactive alternative to the [3H]-thymidine incorporation assay.

Spécificité

The antibody conjugate reacts with the thymidine analogue 5-bromo-2′-deoxyuridine (BrdU) and with BrdU incorporated into DNA. For binding to BrdU incorporated into the DNA, the BrdU-labeled DNA has to be denatured. The antibody does not cross-react with any endogenous cellular components such as thymidine, uridine, or DNA.

Application

Cell Proliferation ELISA, BrdU (chemiluminescent) has been used:
  • for bromodeoxyuridine (BrdU) proliferation assay in ReN and primary astrocytes
  • to test the effect on imatinib on the proliferation of neuroblastoma cells using BrdU incorporation assay
  • to check the effect of palbociclib on cell cycle inhibition using BrdU incorporation in pancreatic patient-derived primary cell lines

The Cell Proliferation ELISA, BrdU (chemiluminescent) belongs to the second, improved generation of kits for measuring DNA synthesis. It is a precise, fast, and simple nonradioactive alternative to quantitate cell proliferation based on the measurement of BrdU incorporation during DNA synthesis in replicating (cycling) cells. Thus, the Cell Proliferation ELISA can be used in many different in vitro cell systems. For example:
  • Detection and quantification of cell proliferation induced by growth factors and cytokines
  • Determination of the inhibitory or stimulatory effects of various compounds on cell proliferation in environmental and biomedical research, and in the food, cosmetic, and pharmaceutical industries
  • Measurement of the immunoreactivity of lymphocytes, stimulated by mitogens or antigens
  • Analysis of the chemosensitivity of tumor cells to different cytostatic drugs in medical research

Conditionnement

1 kit containing 7 components.

Composants

  1. BrdU Labeling Reagent, 1,000x conc
  2. FixDenat
  3. Anti-BrdU-POD 4. Antibody Dilution Solution
  4. Washing Buffer PBS, 10x conc.
  5. Substrate Component A
  6. Substrate Component B

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Composants de kit seuls

Réf. du produit
Description
  • BrdU Labeling Reagent
  • FixDenat ready-to-use
  • Anti-BrdU-peroxidase antibody
  • Antibody Dilution Solution ready-to-use
  • ready-to-use 10x concentrated
  • Substrate Component A
  • Substrate Component B

Pictogrammes

FlameExclamation markHealth hazard
GHS02,GHS07,GHS08

Mention d'avertissement

Danger

Mentions de danger

H225,H317,H319,H340

Classification des risques

Eye Irrit. 2 - Flam. Liq. 2 - Muta. 1B - Skin Sens. 1

Code de la classe de stockage

3 - Flammable liquids

Classe de danger pour l'eau (WGK)

WGK 1

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Mette Lorenzen et al.
Cancers, 12(6) (2020-05-30)
Luteinizing hormone/choriogonadotropin receptor (LHCGR) regulates gonadal testosterone production and recent studies have suggested a growth-regulatory role in somatic cancers. Here, we established that LHCGR is expressed in a fraction of seminoma cells and germ cell neoplasia in situ (GCNIS), and
Diego S D'Astolfo et al.
Cell, 161(3), 674-690 (2015-04-25)
Modulation of protein function is used to intervene in cellular processes but is often done indirectly by means of introducing DNA or mRNA encoding the effector protein. Thus far, direct intracellular delivery of proteins has remained challenging. We developed a
Min Wu et al.
Frontiers in endocrinology, 13, 775278-775278 (2022-05-10)
This study aims to identify reliable prognostic biomarkers for differentiated thyroid cancer (DTC) based on glycolysis-related genes (GRGs), and to construct a glycolysis-related gene model for predicting the prognosis of DTC patients. We retrospectively analyzed the transcriptomic profiles and clinical
Xiao-Feng Wang et al.
Molecular medicine reports, 12(4), 5917-5923 (2015-08-25)
Epirubicin, an anthracycline derivative, is one of the main line treatments for brain tumors. The aim of the present study was to verify that epirubicin alters the growth and morphological characteristics of U‑87 glioma cells. In the present study, the
Valerio Ciccone et al.
Oncotarget, 9(17), 13353-13365 (2018-03-24)
Nitric oxide (NO) exerts conflicting effect on tumor growth and progression, depending on its concentration. We aimed to characterize the anti-cancer activity of a new NO donor, Ni(SalPipNONO) belonging to the class of metal-nonoates, in epithelial derived tumor cells, finally
Afficher tous les articles scientifiques apparentés

Articles

Cell Cycle Analysis Using a Nucleoside Triphosphate (NTP) Transporter Molecule for Rapid DNA Labeling in Living Cells

Regulation of the cell cycle involves processes crucial to the survival of a cell, including the detection and repair of genetic damage as well as the prevention of uncontrolled cell division associated with cancer. The cell cycle is a four-stage process in which the cell 1) increases in size (G1-stage), 2) copies its DNA (synthesis, S-stage), 3) prepares to divide (G2-stage), and 4) divides (mitosis, M-stage). Due to their anionic nature, nucleoside triphosphates (NTPs), the building blocks of both RNA and DNA, do not permeate cell membranes.

Cell Viability and Proliferation Assays

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

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