CELLPRO-RO
Roche
Réactif de prolifération cellulaire WST-1
suitable for protein quantification, suitable for cell analysis, detection, solution
Synonyme(s) :
Réactif de prolifération cellulaire, wst-1
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About This Item
Code UNSPSC :
12352200
Nomenclature NACRES :
NA.21
Produits recommandés
Forme
solution
Niveau de qualité
Utilisation
sufficient for ≤2,500 tests (11644807001)
sufficient for ≤800 tests (05015944001)
Conditionnement
bottle of 25 mL (11644807001)
bottle of 8 mL (05015944001)
Fabricant/nom de marque
Roche
Conditions de stockage
protect from light
Technique(s)
protein quantification: suitable
pH optimal
8.0(for physiological conditions our product is buffered pH 7.3)
λmax
440-480 nm
Application(s)
cell analysis
detection
Méthode de détection
colorimetric
Température de stockage
−20°C
Description générale
Sommaire
Ready-to-use solution, containing WST-1 and an electron coupling reagent.
Ready-to-use solution, containing WST-1 and an electron coupling reagent.
Colorimetric assay (WST-1 based) for the nonradioactive quantification of cell proliferation, cell viability, and cytotoxicity.
La prolifération cellulaire nécessite la réplication de l'ADN génomique. Thus, monitoring DNA synthesis is an indirect parameter of cell proliferation, as well as being suitable for the study of the regulation of DNA synthesis. ′ The use of chemiluminescence technology provides enhanced sensitivity and a broad measurement range.
Application
The Cell Proliferation Reagent WST-1 is used for the nonradioactive, spectrophotometric quantification of cell proliferation and viability in cell populations using the 96-well-plate format. Measurement of cell proliferation in response to growth factors, cytokines, mitogens, and nutrients.
- Analysis of cytotoxic and cytostatic compounds, such as anti-cancer drugs and other pharmaceutical compounds
- Assessment of growth inhibitory antibodies and physiological mediators
Caractéristiques et avantages
- Pratique : Benefit from a ready-to-use reagent.
- Eliminate radioactive isotopes, washing steps, and additional reagents.
- Précis : The absorbance obtained strongly correlates to the cell number.
- Sensible Detect low cell numbers.
- Rapides : Process a large number of samples using a multi-well ELISA reader.
Principe
The stable tetrazolium salt WST-1 is cleaved to a soluble formazan by a complex cellular mechanism that occurs primarily at the cell surface. This bioreduction is largely dependent on the glycolytic production of NAD(P)H in viable cells. Therefore, the amount of formazan dye formed directly correlates to the number of metabolically active cells in the culture.
Cells grown in a 96-well tissue culture plate are incubated with the WST-1 reagent for 0.5 - 4 hours. After this incubation period, the formazan dye formed is quantitated with a scanning multi-well spectrophotometer (ELISA reader). The measured absorbance directly correlates to the number of viable cells.
Cell proliferation and viability assays are of particular importance for routine applications in cell biology. Tetrazolium salts (e.g., MTT, XTT, WST-1) are particularly useful for this type of analysis. Tetrazolium salts are cleaved to formazan by the succinate-tetrazolium reductase system (EC 1.3.99.1) which belongs to the respiratory chain of the mitochondria, and is only active in metabolically intact cells.
Cells grown in a 96-well tissue culture plate are incubated with the WST-1 reagent for 0.5 - 4 hours. After this incubation period, the formazan dye formed is quantitated with a scanning multi-well spectrophotometer (ELISA reader). The measured absorbance directly correlates to the number of viable cells.
Cell proliferation and viability assays are of particular importance for routine applications in cell biology. Tetrazolium salts (e.g., MTT, XTT, WST-1) are particularly useful for this type of analysis. Tetrazolium salts are cleaved to formazan by the succinate-tetrazolium reductase system (EC 1.3.99.1) which belongs to the respiratory chain of the mitochondria, and is only active in metabolically intact cells.
Notes préparatoires
Concentration de travail : μμ
μ
μμ
When precipitates or turbidity are observed upon thawing, warm up the solution to 37 °C for 2 to 10 minutes and agitate to dissolve the precipitates.Centrifugation is not recommended because the working concentration would decrease. After being dissolved, the WST-1 reagent can be used without any limitations.
However please note that the solution may become viscous.
μ
μμ
When precipitates or turbidity are observed upon thawing, warm up the solution to 37 °C for 2 to 10 minutes and agitate to dissolve the precipitates.Centrifugation is not recommended because the working concentration would decrease. After being dissolved, the WST-1 reagent can be used without any limitations.
However please note that the solution may become viscous.
Autres remarques
Pour la recherche en sciences de la vie uniquement. Ne pas utiliser dans des procédures de diagnostic.
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
nwg
Point d'éclair (°F)
No data available
Point d'éclair (°C)
No data available
Certificats d'analyse (COA)
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Articles
Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.
Protocoles
WST-1 assay protocol for measuring cell viability, proliferation, activation and cytotoxicity. Instructions for WST-1 reagent preparation and examples of applications. Frequently asked questions and troubleshooting guide for WST-1 assay.
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