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10109878001

Roche

Trypsin Inhibitor

from chicken egg white

Synonyme(s) :

trypisin

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About This Item

Code UNSPSC :
12352207

Source biologique

chicken egg white

Niveau de qualité

100

Forme

powder

Conditionnement

pkg of 1 g

Fabricant/nom de marque

Roche

Concentration

1 mg/mL

Technique(s)

cell culture | mammalian: suitable

pH optimal

3.8-4.5

Solubilité

water: soluble

Température de stockage

2-8°C

Description générale

Egg white trypsin inhibitor is an inhibitor for trypsin, plasmin and plasma kallikrein activity in serum-free cell-culture media. It does not inhibit metallo-, cysteine, and aspartic proteases or tissue kallikrein (serine protease).

Application

Chicken egg Trypsin Inhibitor is used for the termination of tissue disaggregation and for subcultivation procedures.
Trypsin Inhibitor has been used:
  • as a component of lysis buffer to treat Tsc2fl/fl (control) and Tsc2fl/flLyz2-Cre (knockout [KO]) bone marrow-derived macrophages (BMDMs) before immunoblotting
  • to inactivate trypsin post detachment of the stem cells in ventricular zone cell culture
  • to neutralize trypsin post dissociation and trypsinization of brain tissues
  • termination of tissue disaggregation and for subcultivation procedures

Notes préparatoires

Working concentration: 0
Working solution: Solvent is recommended in distilled water.
Stock solution: 1 mg/ml
Storage conditions (working solution): -15 to -25 °C
Stable for at least 6 months in distilled water or 1 mM HCI when stored frozen in aliquots. Avoid repeated freeze-thaw cycles.

Reconstitution

Both are soluble in water. Recommended stock solution: 1 mg/ml. Store frozen in aliquots at -15 to -25 °C. Stable for at least 6 months.

Stockage et stabilité

Store at 2 to 8 °C. (Store dry!)

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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Maksim Sinyuk et al.
Journal of visualized experiments : JoVE, (160) (2020-06-23)
The methods presented here demonstrate laboratory procedures for the dissection of four different regions of the central nervous system (CNS) from murine neonates for the isolation of glial subpopulations. The purpose of the procedure is to dissociate microglia, oligodendrocyte progenitor
Haley M Peckham et al.
Journal of visualized experiments : JoVE, 95(95), 52179-52179 (2015-02-05)
Myelination is a complex process that involves both neurons and the myelin forming glial cells, oligodendrocytes in the central nervous system (CNS) and Schwann cells in the peripheral nervous system (PNS). We use an in vitro myelination assay, an established
Membrane properties and electrogenesis in the distal axons of small dorsal root ganglion neurons in vitro.
Vasylyev DV and Waxman SG
Journal of Neurophysiology, 108(3), 729-740 (2012)
Nikesh Kunder et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 42(49), 9129-9141 (2022-10-22)
HuR is an RNA-binding protein implicated in RNA processing, stability, and translation. Previously, we examined protein synthesis in dorsal root ganglion (DRG) neurons treated with inflammatory mediators using ribosome profiling. We found that the HuR consensus binding element was enriched
Leandro Castaneyra-Ruiz et al.
Fluids and barriers of the CNS, 17(1), 46-46 (2020-07-22)
Severe intraventricular hemorrhage (IVH) is one of the most devastating neurological complications in preterm infants, with the majority suffering long-term neurological morbidity and up to 50% developing post-hemorrhagic hydrocephalus (PHH). Despite the importance of this disease, its cytopathological mechanisms are

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