NB20

Anti-Ankyrin_G Mouse mAb (463)

liquid, ≥95% (SDS-PAGE), clone 463

• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 463, monoclonal
• Essai: ≥95% (SDS-PAGE)
• Forme: liquid
• Contient: ≤0.1% sodium azide as preservative
• Espèces réactives: rat, mouse, human, canine
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: do not freeze
• Isotype: IgG1
• Conditions d'expédition: wet ice
• Température de stockage: 2-8°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... ANK3(288)

Description

Description générale

Anti-Ankyrin G, mouse monoclonal, clone 463, recognizes the ~190 kDa ankyrin G protein in brain extracts. It is validated for Western blotting, immunofluorescence, and frozen sections.

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3X63/Ag 8.653 mouse myeloma cells. Recognizes the ~190 kDa isoform of Ankyrin_G.

Recognizes the ~190 kDa ankyrin_G protein in brain extracts. Reacts with all spliced forms of ankyrin_G.

Antibody Target Gene Symbol: ANK3

Target Synonym: 2900054D09RIK, AI314020, AnkG, Ankyrin-3, ANKYRIN-G, FLJ45464, MGC14049

Entrez Gene Name: ankyrin 3, node of Ranvier (ankyrin G)

Hu Entrez ID: 288 (Related Antibodies: AP1034, PC523, PC522, AP1057)

Mu Entrez ID: 11735

Rat Entrez ID: 361833

Immunogène

Human

a synthetic peptide corresponding to amino acids from the spectrin-binding domain of human ankyrin_G

Application

Frozen Sections (1-2 g/ml)
Immunoblotting (2-5 g/ml)
Immunofluorescence (1-2 g/ml)

Conditionnement

Please refer to vial label for lot-specific concentration.

Avertissement

Toxicity: Standard Handling (A)

Forme physique

In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.

Remarque sur l'analyse

Positive Control
Mouse or rat brain extract or unmyelinated axons

Autres remarques

Ankyrin_G is a good immunohistochemical marker for axon initial segments, nodes of Ranvier, and epithelial cell-cell contact sites. Antibody should be titrated for optimal results in individual systems.

Davis, J.Q., et al. 1995. Mol. Biol. Cell6, 177a, supplement.
Kunimoto, M., et al. 1995. FEBS Lett.357, 217.
Lambert, S., et al. 1995. Mol. Biol. Cell6, 98a supplement.
Bennett, V. and Gilligan, D.M. 1993. Ann. Rev. Cell Biol.9, 27.
Kunimoto, M., et al. 1991. J. Cell Biol.115, 1319.
Otto, E., et al. 1991. J. Cell Biol.114, 241.
Davis, J., and Bennett, V. 1984. J. Biol. Chem.259, 13550.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable