MABT140

Anti-Claudin-4 Antibody, clone 10B4.3

clone 10B4.3, from mouse

• Synonyme(s) : Claudin-4, Clostridium perfringens enterotoxin receptor, CPE-R, CPE-receptor, Williams-Beuren syndrome chromosomal region 8 protein
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 10B4.3, monoclonal
• Espèces réactives: human
• Technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• Isotype: IgG1κ
• Numéro d'accès NCBI: NP_001296
• Numéro d'accès UniProt: O14493
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... CLDN4(1364)

Description

Description générale

Claudin 4, also known as Clostridium perfringens enterotoxin receptor, or CPE-R, or CPE-receptor or Williams-Beuren syndrome chromosomal region 8 protein, and encoded by the gene CLDN4/CPER/CPETR1/WBSCR8, plays a critical role in tight junction specific obliteration of intercellular space. Claudin 4 directly interacts with several important tight junction associated proteins including ZO-1,2,3 and EPHA2 proteins, and through these interactions, Claudin 4 regulates tight junction formation. Claudin 4 is localized to cell junctions and membranes at tight junctions. Claudin 4 is expressed in most tissues particularly in intestine, liver, and pancreas. Defects in Claudin 4 may be associated with Williams Beuren syndrome genetic deletion syndrome. Claudin 4 is also over expressed in many cancers and thus is a target of anti-cancer research using C. perfringens enterotoxin.

Immunogène

Epitope: Cytoplasmic domain

KLH-conjugated linear peptide corresponding to the cytoplasmic domain of human Claudin-4.

Application

Anti-Claudin-4 Antibody, clone 10B4.3 is an antibody against Claudin-4 for use in western blotting & IHC (Paraffin).

Research Category
Cell Structure

Research Sub Category
ECM Proteins

Western Blotting Analysis: 2.0 µg/mL from a representative lot detected Claudin-4 in 10 µg of MCF-7 cell lysate.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Claudin-4 in human ileum and human tonsil tissue.

Qualité

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected Claudin-4 in 10 µg of HeLa cell lysate.

Description de la cible

~ 22 kDa observed

Forme physique

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable