MABN1182
Anti-Glutamine Synthetase Antibody, clone 11A3.1
clone 11A3.1, from mouse
Synonyme(s) :
Glutamine synthetase, Glutamate-ammonia ligase, Glutamate decarboxylase, GS
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
11A3.1, monoclonal
Espèces réactives
human, rat
Technique(s)
immunohistochemistry: suitable (paraffin)
western blot: suitable
Isotype
IgG2aκ
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
ambient
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... GLUL (2752)
rat ... Glul(24957)
Description générale
Glutamine synthetase (EC 4.1.1.15, EC 6.3.1.2; UniProt P09606; also known as Glutamate-ammonia ligase, Glutamate decarboxylase, GS) is encoded by the Glul (also known as Glns) gene (Gene ID 24957) in rat species. Glutamine synthetase (GS) plays an essential role in nitrogen metabolism by catalyzing ATP-dependent condensation of glutamate and ammonia to form glutamine. Three classes of GS exist, class I enzymes (GSI) are specific to prokaryotes and exist as oligomers of 12 identical subunits, class II enzymes (GSII) exist as decamer of identical subunits in eukaryotes and in some bacteria, while class III enzymes (GSIII) function as a double-ringed dodecamer of identical chains found in bacteroides fragilis and in butyrivibrio fibrisolvens. GS is present predominantly in the brain, kidneys, and liver in mammals. In the brain, GS is found primarily in astrocytes, where it participates in the the detoxification of ammonia via assimilation and termination of neurotransmitter signals via recyclization. c-Myc promotes glutamine usage by upregulating glutaminase (GLS), which converts glutamine to glutamate in the TCA cycle, while in some cancer cells Myc-mediated thymine DNA glycosylase (TDG) transcription leads to GS upregulation due to TDG-dependent demethylation of the GS promoter. GS upregulation promotes cancer cell survival under glutamine limitation, while GS silencing decreases cancer cell proliferation and xenograft tumor growth.
Spécificité
Clone 11A3.1 targets an internal epitope within the C-terminal half of human and rat glutamine synthetase.
Immunogène
GST-tagged recombinant rat glutamine synthetase internal fragment.
Application
Anti-Glutamine Synthetase, clone 11A3.1, Cat. No. MABN1182, is a highly specific mouse monoclonal antibody that targets Glutamine Synthetase, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.
Research Category
Neuroscience
Neuroscience
Western Blotting Analysis: A 1:20,000 dilution of from a representative lot detected Glutamine synthetase in 10 µg of human brain tissue lysate.
Immunohistochemistry Analysis: A 1:1000 dilution from a representative lot detected Glutamine synthetase in human cerebellum, cerebral cortex, and tonsil tissue sections.
Immunohistochemistry Analysis: A 1:1000 dilution from a representative lot detected Glutamine synthetase in human cerebellum, cerebral cortex, and tonsil tissue sections.
Qualité
Evaluated by Western Blotting in rat brain tissue lysate.
Western Blotting Analysis: A 1:20,000 dilution of this antibody detected Glutamine synthetase in 10 µg of rat brain tissue lysate.
Western Blotting Analysis: A 1:20,000 dilution of this antibody detected Glutamine synthetase in 10 µg of rat brain tissue lysate.
Description de la cible
~42 kDa observed. 42.06 kDa (human) and 42.27 kDa (rat) calculated. Uncharacterized bands may be observed in some lysate(s).
Forme physique
Format: Purified
Protein G purified.
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4) 150 mM NaCl with 0.05% sodium azide
Stockage et stabilité
Stable for 1 year at 2-8°C from date of receipt.
Autres remarques
Concentration: Please refer to lot specific datasheet.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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