Accéder au contenu
Merck
Toutes les photos(1)

Key Documents

MABF2689

Sigma-Aldrich

Anti-NKG2A/C/E Antibody

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme


About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Le tarif et la disponibilité ne sont pas disponibles actuellement.

Source biologique

rat

Niveau de qualité

Conjugué

unconjugated

Forme d'anticorps

purified antibody

Type de produit anticorps

primary antibodies

Clone

20D5, monoclonal

Poids mol.

observed mol wt ~27.68 kDa

Espèces réactives

mouse

Conditionnement

antibody small pack of 100 μL

Technique(s)

flow cytometry: suitable
inhibition assay: suitable

Comparer avec des articles similaires

Voir la comparaison complète

Montrer les différences

1 of 4

Cet article
MABF2237SAB1406063SAB1410734
antibody form

purified antibody

antibody form

purified antibody

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

biological source

rat

biological source

mouse

biological source

mouse

biological source

rabbit

Gene Information

mouse ... Klrc1(16641)

Gene Information

human ... NCR3(259197)

Gene Information

human ... KLRC1(3821)

Gene Information

human ... KLRC1(3821)

clone

20D5, monoclonal

clone

1C01, monoclonal

clone

polyclonal

clone

polyclonal

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

Description générale

Killer cell lectin-like receptor subfamily C, member 1/2/3 (UniProt: Q9Z202/ Q9WTJ9/Q9QXN7; also known as Natural killer cell protein group 2-A1/ Natural killer cell protein group 2-C1, Natural killer cell receptor NKG2A/Natural killer cell receptor NKG2C/ Natural killer cell group 2E cell receptor NKG2E) are encoded by the Klrc1/2/3 genes (Gene IDs: 16641/ 16642/58179) in murine species. The NKG2 proteins are members of the lectin-like receptor family that heterodimerize with CD94 on the surface of NK and NKT cells. They are also shown to be expressed on activated CD8+ T cells. They are integral component of membrane and are present on the external side of plasma membrane. Within their extracellular carbohydrate recognition domains, NKG2A, NKG2C and NKG2E share extensive homology; however, NKG2C and NKG2E receptors differ significantly from NKG2A in their cytoplasmic domains. NKG2/CD94 heterodimeric complexes are shown to recognize Qa-1 that is described as a nonclassical MHC class I antigen. These heterodimers are reported to transduce signals following ligand binding. NKG2A is shown to be inhibitory in nature, while NKG2C and NKG2E transduce stimulatory signals. Comparatively, NKG2A is more abundant than NKG2C and NKG2E in NK cells. Murine NKG2A contains two intracytoplasmic sequences that closely resemble the Immunoreceptor Tyrosine- based Inhibitory Motif (ITIM). Clone 20D5 can be used for the identification of NK cells expressing functional CD94/NKG2 receptors and is suitable for blocking NKG2A signals in murine tumor models. (Ref.: Vance, RE., et al. (1999). J. Exp. Med. 190(12); 1801-1812).

Spécificité

Clone 20D5 is a rat monoclonal antibody that detects murine Killer cell lectin-like receptor subfamily C (NKG2 A, C and E). It targets an epitope within the extracellular domain.

Immunogène

Stable Chinese hamster ovary (CHO) cells expressing the B6 alleles of CD94 and NKG2A.

Application

Quality Control Testing

Evaluated by Flow Cytometry in C57BL/6 mouse splenocytes.

Flow Cytometry Analysis: 1 μg of this antibody detected NKG2A/C/E in one million C57BL/6 mouse splenocytes.

Tested Applications

Flow Cytometry Analysis: A representative lot detected NKG2A/C/E in Flow Cytometry applications (Vance, R.E., et. al. (1999). J Exp Med. 190(12):1801-12).

Inhibition Analysis: A representative lot of this antibody completely blocked all binding of Qa-1 tetramers to NK1.11 splenocytes. (Vance, R.E., et. al. (1999). J Exp Med. 190(12):1801-12).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-NKG2A/C/E, clone 20D5, Cat. No. MABF2689, is a rat monoclonal antibody that detects Killer cell lectin-like receptor subfamily C, member 1/2/3 and is tested for use in Flow Cytometry and Inhibition Assay.

Forme physique

Purified rat monoclonal antibody IgG2a in PBS without azide.

Stockage et stabilité

Stable for 1 year at -10°C to -25°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Vous ne trouvez pas le bon produit ?  

Essayez notre Outil de sélection de produits.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


  • Certificats d'analyse (COA)

    Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

    Déjà en possession de ce produit ?

    Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

    Consulter la Bibliothèque de documents

    Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

    Contacter notre Service technique