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Key Documents

MAB5446

Sigma-Aldrich

Anti-Neurofilament NF-H Antibody, tail domain (Phosphate dependent), clone RMO-24

ascites fluid, clone RMO-24, Chemicon®

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

Forme d'anticorps

ascites fluid

Type de produit anticorps

primary antibodies

Clone

RMO-24, monoclonal

Espèces réactives

human, rat

Fabricant/nom de marque

Chemicon®

Technique(s)

ELISA: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

Isotype

IgG1κ

Numéro d'accès NCBI

Numéro d'accès UniProt

Conditions d'expédition

dry ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... NEFH(4744)
rat ... Nefh(24587)

Spécificité

Reacts with Neurofilament Heavy (NF-H), tail domain (phosphate dependent). Dephosphorylation of the NF-H will results in diminished immunoreactivity.

Immunogène

Epitope: tail domain (Phosphate dependent)
Rat Neurofilaments

Application

Anti-Neurofilament NF-H Antibody, tail domain (Phosphate dependent), clone RMO-24 detects level of Neurofilament NF-H & has been published & validated for use in ELISA, IP, WB, IH.
Research Category
Neuroscience
Research Sub Category
Neurofilament & Neuron Metabolism

Neuronal & Glial Markers
Western blot. Reacts with the 200 kD NF-H from human.

Immunohistochemistry. The antibody has been used successfully on Bouin′s and alcohol-fixed paraffin-embedded or frozen tissue sections. Formalin-fixed paraffin-embedded tissue sections require pretreatment with trypsin.

Immunoprecipitation

Optimal working dilutions must be determined by end user.

Forme physique

Ascites fluid. Liquid.

Stockage et stabilité

Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.

Remarque sur l'analyse

Control
Positive Control: Brain

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Hirofumi Tokuoka et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 26(45), 11606-11614 (2006-11-10)
The mechanism by which synaptic vesicles (SVs) are recruited to the release site is poorly understood. One candidate mechanism for trafficking of SVs is the myosin-actin motor system. Myosin activity is modulated by myosin light chain kinase (MLCK), which in
V M Lee et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 7(11), 3474-3488 (1987-11-01)
A new panel of greater than 300 monoclonal antibodies (mAbs) was prepared to the high, middle, and low Mr rat neurofilament (NF) subunits (NF-H, NF-M and NF-L, respectively). NF proteins were purified both from native, i.e., phosphorylated rat NFs and

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