N4142

Anti-Neurofilament 200 antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Synonyme(s) : NF200 Antibody - Anti-Neurofilament 200 antibody produced in rabbit, Nf200 Antibody, AntiNeurofilament heavy chain
• Numéro MDL: MFCD02263444
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 200
• Conjugué: unconjugated
• Forme d'anticorps: IgG fraction of antiserum
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen 200 kDa
• Espèces réactives: bovine, wide range
• Conditionnement: antibody small pack of 25 μL
• Validation améliorée: independent ( Antibodies); Learn more about Antibody Enhanced Validation
• Technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:80 using animal cerebellum sections; microarray: suitable; western blot: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... NEFH(4744) ; mouse ... Nefh(380684) ; rat ... Nefh(24587)

Description

Description générale

Neurofilament 200, also known as neurofilament heavy polypeptide (NEFH), is encoded by the gene mapped to human chromosome 22q12.2. The encoded protein has a molecular mass of 200kDa and belongs to the group of type IV intermediate filaments which are key constituents of the neuronal cytoskeleton. NEFH contains C-terminal motifs involved in the formation of sidearm projections which cross-link the neurofilaments.

Spécificité

Shows a wide species cross-reactivity. Reacts with neurofilaments in the central and peripheral nervous system and localizes the neurofilament polypeptide.

Immunogène

Neurofilament 200 from bovine spinal cord.

Application

Anti-neurofilament 200 antibody produced in rabbit has been used for immunolabeling.
Anti-neurofilament 200 antibody produced in rabbit has been used:

• for detection of neurofilaments using immunohistological staining
• in human astrocytoma cell line
• in post-mortem brain sections
• in human cremaster muscles
• for detection of neurofilaments by immunofluorescence staining in lumbar spinal cord sections of mice

Actions biochimiques/physiologiques

Neurofilament 200 plays a vital role in cytoarchitecture organization. Downregulated expression of the gene has been observed in schizophrenia patients. Loss of gene expression is associated with the development of prostate carcinoma. Mutation in the gene leads to sporadic amyotrophic lateral sclerosis (ALS).
Neurofilament 200 assembly during development is critical for normal functioning of neuronal cells. Mutations in gene encoding neurofilaments is associated with neurological disorder called the Charcot–Marie–Tooth.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Stockage et stabilité

Store at -20°C. For continuous use, the product may be stored at 2-8°C for up to one month. For extended storage, solution may be frozen in working aliquots at –20°C. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable