MAB5256X
Anti-Neurofilament H Antibody, clone NE14, Alexa Fluor™ 488 Conjugated
clone NE14, from mouse, ALEXA FLUOR™ 488
Synonyme(s) :
200 kDa neurofilament protein, Neurofilament triplet H protein, neurofilament, heavy polypeptide, neurofilament, heavy polypeptide 200kDa
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Niveau de qualité
Conjugué
ALEXA FLUOR™ 488
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
NE14, monoclonal
Espèces réactives
human, rat, pig, porcine
Technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
Isotype
IgG1
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... NEFH(4744)
pig ... Nefh(100156492)
rat ... Nefh(24587)
Description générale
Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexes of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure; however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments.
Spécificité
Reactivity with other species has not been tested.
This antibody recognizes Neurofilament H.
Immunogène
Epitope: Unknown
Purified neurofilament polypeptides (Debus et al., 1983).
Application
Anti-Neurofilament H Antibody, clone NE14, Alexa Fluor 488 Conjugated is an antibody against Neurofilament H for use in IH & IC.
Immunohistochemistry:
5-10 µg/mL on frozen brain tissue. Optimal working dilutions must be determined by end user.
5-10 µg/mL on frozen brain tissue. Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurofilament & Neuron Metabolism
Neurofilament & Neuron Metabolism
Qualité
Immunocytochemical Analysis: 1:250 dilution of this lot detected Neurofilament-H in rat cortex cell culture.
Description de la cible
~ 200 kDa
Forme physique
Protein G Purified
Purified immunoglobulin in 0.02M Phosphate buffer, 0.25M NaCl containing 0.1% sodium azide.
Stockage et stabilité
Stable for 6 months at 2-8ºC from date of receipt.
Remarque sur l'analyse
Control
Rat Cortex Cells.
Rat Cortex Cells.
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informations légales
ALEXA FLUOR is a trademark of Life Technologies
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Sienna S Drake et al.
International journal of molecular sciences, 23(23) (2022-12-12)
Multiple sclerosis (MS) is an autoimmune and neurodegenerative disease driven by inflammation and demyelination in the brain, spinal cord, and optic nerve. Optic neuritis, characterized by inflammation and demyelination of the optic nerve, is a symptom in many patients with
Sandeep Sheth et al.
Scientific reports, 9(1), 9571-9571 (2019-07-04)
Adenosine A1 receptors (A1AR) are well characterized for their role in cytoprotection. Previous studies have demonstrated the presence of these receptors in the cochlea where their activation were shown to suppress cisplatin-induced inflammatory response and the resulting ototoxicity. Inhibition of
S S Oh et al.
Neuroscience, 212, 201-213 (2012-04-21)
The contribution of oxidative stress to diabetic complications including neuropathy is widely known. Mitochondrial and cellular damage are associated with the overproduction of reactive oxygen species and decreased levels or function of the cellular antioxidant mitochondrial manganese superoxide dismutase (SOD2).
Hiromi Nakai-Shimoda et al.
iScience, 25(1), 103609-103609 (2022-01-11)
Glucose-responsive ATP-sensitive potassium channels (KATP) are expressed in a variety of tissues including nervous systems. The depolarization of the membrane potential induced by glucose may lead to hyperexcitability of neurons and induce excitotoxicity. However, the roles of KATP in the
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