MAB3785

Anti-APC Antibody, NT, clone Ali 12.28

clone Ali 12.28, Chemicon^®, from mouse

• Synonyme(s) : Adenomatous Polyposis Coli Protein
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: Ali 12.28, monoclonal
• Espèces réactives: human
• Ne doit pas réagir avec: mouse
• Fabricant/nom de marque: Chemicon^®
• Technique(s): ELISA: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotype: IgG1
• Numéro d'accès NCBI: NM_000038.3
• Numéro d'accès UniProt: P25054
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... APC(324) ; mouse ... Apc(11789)

Description

Description générale

The adenomatous polyposis coli tumor suppressor gene is mutated (often deletion of the C-terminal portion of APC) in the inherited disease, familial adenomatous polyposis (FAP), and over 80% of colorectal cancers. Ali 12-28 can be used for APC expression and detection of APC mutations.

Spécificité

Recognizes human APC, molecular weight ~310 kDa, as well as C-terminal truncation mutants containing the epitope mapped between amino acids 135 to 422.

Immunogène

Epitope: N-terminus

N-terminal APC-MBP fusion protein (first 1.3 kb of APC which includes the first 433 aa and the first 9 exons of the gene).

Application

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Use Anti-APC Antibody, N-terminus, clone Ali 12.28 (Mouse Monoclonal Antibody) validated in ELISA, IP, WB, IHC to detect APC also known as Adenomatous Polyposis Coli Protein.

Western blot: 4 μg/mL

Immunohistochemistry (Acetone Fixed, Frozen)

ELISA

Immunoprecipitation

Optimal working dilutions must be determined by end user.

Liaison

Replaces: AB1268

Forme physique

Format: Purified

Purified immunoglobulin. Liquid in 0.02M phosphate buffer, 0.25M NaCl, pH 7.6, with 0.1% sodium azide.

Stockage et stabilité

Maintain at 2-8°C in undiluted aliquots for up to 12 months from date of receipt.

Remarque sur l'analyse

Control
POSITIVE CONTROL: SW480, LoVo, DLD1, and SW837 cell lines

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable