MAB1957Z

Anti-Integrin β3 Antibody, clone 25E11, azide free

clone 25E11, Chemicon^®, from mouse

• Synonyme(s) : CD61, MAB1957
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 25E11, monoclonal
• Espèces réactives: human
• Fabricant/nom de marque: Chemicon^®
• Technique(s): flow cytometry: suitable; immunocytochemistry: suitable; immunoprecipitation (IP): suitable
• Isotype: IgM
• Numéro d'accès NCBI: NM_000212.2
• Numéro d'accès UniProt: P05106
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... ITGB3(3690)

Description

Description générale

The integrin family of cell adhesion receptors consists of several membrane-associated heterodimers, composed of an alpha and beta subunit that associate in a non-covalent manner. The structure and functional diversity of the integrin family are based upon the pairing abilities of the individual alpha and beta subunits. Key to these molecular interactions between the integrin receptors and their respective ligands is the recognition of the Arg-Gly-Asp (RGD) sequence, known to be present in the extracellular matrix components fibronectin, vitronectin, collagen, fibrinogen, and von Willebrand factor. The involvement of integrins in vascular proliferation, adhesion, and wound repair has been well documented.
Platelet membrane glycoprotein Integrin beta3 or CD61 (GP IIIa) forms a Ca2+-dependent heterodimer complex with GP IIb. The GP IIb-IIIa complex constitutes the fibrinogen and fibronectin receptor on stimulated platelets.

Spécificité

Reacts with human integrin beta3 (GPIIIa, vitronectin receptor beta chain). The protein detectable is a complex of CD41 and CD61. Ligands are fibronectin, fibrinogen, von Willebrand factor, vitronectin and thrombospondin. Residues 237-248 of GPIIIa or CD61 are critical in adhesive protein binding. The monocytoid cell line U937 is highly positive.

(original paper claimed IgG2ak)

Immunogène

Normal blood mononuclear cells activated by mixed lymphocyte reaction and cultured for 10 days in medium containing IL-2.

Application

Detect Integrin β3 using this Anti-Integrin β3 Antibody, clone 25E11, azide free validated for use in FC, IP & IC.

Immunoprecipitation

Immunocytochemistry (acetone fixation required).

Flow cytometry

Inhibits platelet aggregation, 10 μg/mL

Optimal working dilutions must be determined by end user.

Research Category
Cell Structure

Research Sub Category
Integrins

Forme physique

Format: Purified

In buffer of 0.01M phosphate, 0.15M sodium chloride, pH 7.1, containing no preservatives.

Stockage et stabilité

Maintain at 2-8°C.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable