ABS88

Anti-phospho-mTOR (Thr2164) Antibody

serum, from rabbit

• Synonyme(s) : Serine/threonine-protein kinase mTOR, FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, Mammalian target of rapamycin, mTOR, Mechanistic target of rapamycin, Rapamycin and FKBP12 target 1, Rapam
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: serum
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Espèces réactives: human
• Technique(s): dot blot: suitable; western blot: suitable
• Numéro d'accès NCBI: NP_004949
• Numéro d'accès UniProt: P42345
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: phosphorylation (pThr2164)
• Informations sur le gène: human ... MTOR(2475)

Description

Description générale

The mammalian target of rapamycin (mTOR), also known as FRAP, RAFT, or RAPT, is a ubiquitously expressed serine/threonine kinase that belongs to the phosphoinositide 3-kinase–related kinase (PIKK) family. mTOR functions as a downstream effector in the PI3K pathway and is activated by the Akt kinase. mTOR responds to signals from growth factors and promotes protein synthesis underlying cell proliferation, by activation of the S6 kinase and inhibition of the 4E-BP protein involved in protein translation. Several studies have suggested that the proliferative functions of mTOR may contribute to the development of cancer.

Spécificité

This antibody recognizes mTOR phosphorylated at Thr2164.

Immunogène

Epitope: Phosphorylated Thr2164

KLH-conjugated linear peptide corresponding to human mTOR phosphorylated at Thr2164.

Application

Anti-mTOR Antibody is an antibody against phospho-mTOR (Thr2164) for use in Dot Blot, Western Blotting.

Research Category
Signaling

Research Sub Category
Kinases & Phosphatases

Western Blot Analysis: A representative lot was used by an independent laboratory in HEK293 cell lysate. (Ekim, B., et al. (2011). MCB. [Epub ahead of print].)

Qualité

Dot Blot Specificity Analysis: Unmodified and phosphorylated mTOR peptides were probed with Anti-phospho-mTOR (Thr2164) (1:1,000 dilution).

Description de la cible

289 kDa calculated

Forme physique

Rabbit polyclonal serum containing 0.05% sodium azide.

Unpurified

Stockage et stabilité

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Remarque sur l'analyse

Control
Unmodified and phosphorylated mTOR peptides

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1