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Merck
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Principaux documents

69026

Millipore

T7•Tag® Antibody Agarose

Synonyme(s) :

T7 Tag Antibody

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About This Item

Code UNSPSC :
41106500
Nomenclature NACRES :
NA.56
application:
citations:
8

Forme

liquid

Niveau de qualité

Fabricant/nom de marque

Novagen®

Conditions de stockage

do not freeze

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Description générale

The T7•Tag Antibody Agarose is designed for rapid immunoaffinity purification of target proteins that carry the T7•Tag sequence (i.e., the amino terminal 11 aa of the T7 gene 10 protein). Purification is based on binding target proteins to T7•Tag monoclonal antibody that is covalently coupled to cross-linked agarose beads, washing away unbound proteins, and eluting at pH 2.2. Capacity will vary somewhat between different target proteins, but the beads are standardized to bind a minimum of 300 µg T7•Tag β-galactosidase per milliliter of settled resin. The beads can be used in either batch or column methods and can be recycled a minimum of five times without loss of binding activity.

Avertissement

Toxicity: Standard Handling (A)

Informations légales

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
T7-Tag is a registered trademark of Merck KGaA, Darmstadt, Germany

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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Satoshi Hirano et al.
Autophagy, 12(2), 312-326 (2016-02-24)
MAP1LC3/LC3 (a mammalian ortholog family of yeast Atg8) is a ubiquitin-like protein that is essential for autophagosome formation. LC3 is conjugated to phosphatidylethanolamine on phagophores and ends up distributed both inside and outside the autophagosome membrane. One of the well-known
Subhashini Sadasivam et al.
Genes & development, 26(5), 474-489 (2012-03-07)
Cell cycle progression is dependent on two major waves of gene expression. Early cell cycle gene expression occurs during G1/S to generate factors required for DNA replication, while late cell cycle gene expression begins during G2 to prepare for mitosis.
Andres López-Perrote et al.
eLife, 9 (2020-11-19)
Nonsense-mediated mRNA decay (NMD) is a surveillance pathway that degrades aberrant mRNAs and also regulates the expression of a wide range of physiological transcripts. RUVBL1 and RUVBL2 AAA-ATPases form an hetero-hexameric ring that is part of several macromolecular complexes such
Shi-You Jiang et al.
PLoS genetics, 15(7), e1008289-e1008289 (2019-07-20)
Schnyder corneal dystrophy (SCD) is a rare genetic eye disease characterized by corneal opacification resulted from deposition of excess free cholesterol. UbiA prenyltransferase domain-containing protein-1 (UBIAD1) is an enzyme catalyzing biosynthesis of coenzyme Q10 and vitamin K2. More than 20

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