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48-610MAG

Millipore

MILLIPLEX MAP STAT Cell Signaling Magnetic Bead 5-Plex Kit - Cell Signaling Multiplex Assay

Synonyme(s) :

Magnetic Bead STAT Kit, STAT Cell Signaling Kit

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About This Item

Code UNSPSC :
12161503
eCl@ss :
32161000
Nomenclature NACRES :
NA.47

Espèces réactives

human

Niveau de qualité

200

Fabricant/nom de marque

Milliplex®

Technique(s)

multiplexing: suitable

Méthode de détection

fluorometric (Luminex xMAP)

Température de stockage

2-8°C

Description générale

The STAT (Signal Transducers and Activators of Transcription) family, consisting of seven mammalian members, regulates many aspects of growth, survival and differentiation in cells. The extracellular binding of cytokines, hormones, growth factors and neurotransmitters to receptors induces phosphorylation of the intracellular Janus kinases (JAKs) that, in turn, phosphorylate STAT proteins at conserved tyrosine residues, activating SH2-mediated dimerization followed by translocation to the nucleus. STAT dimers then bind to IRE (interferon response element) and GAS (gamma interferon-activated sequence) DNA elements, resulting in the transcriptional regulation of downstream genes.

Spécificité

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Application

  • An overnight (4°C) incubation is recommended for best results.
  • This assay requires 25 μL diluted cell lysate per well.
  • This kit must be run using Assay Buffer 1 (provided).
  • 1 - 25 μg cell lysate/well (recommended starting concentration is 40 to 1,000 μg protein/mL).

Research Category
Signaling|Apoptosis & Cancer
The analytes available for this multiplex kit are: STAT1 (Tyr701), STAT2 (Tyr690), STAT3 (Tyr705), STAT5A/B (Tyr694/699), STAT6 (Tyr641).
Used to detect/quantify:STAT1 (Tyr701)|STAT2 (Tyr690)|STAT3 (Tyr705)|STAT5A/B (Tyr694/699)|STAT6 (Tyr641)

Conditionnement

96-well plate

Stockage et stabilité

Recommended storage for kit components is 2 - 8°C.

Informations légales

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

Pictogrammes

CorrosionExclamation markEnvironment
GHS05,GHS07,GHS09

Mention d'avertissement

Danger

Mentions de danger

H302,H315,H318,H410

Classification des risques

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2

Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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