1.04157

Fluorescein-5-isothiocyanate (FITC)

• Formule empirique (notation de Hill): C₂₁H₁₁NO₅S
• Numéro CAS: 3326-32-7
• Poids moléculaire : 389.38
• Numéro MDL: MFCD00005063
• Code UNSPSC : 41116130
• Nomenclature NACRES : NA.21

Propriétés

• Composition: Dye, ≥95% spectrophotometric assay
• Niveau de qualité: 200
• Pf: >360 °C
• Application(s): diagnostic assay manufacturing; hematology; histology
• Température de stockage: 2-8°C
• InChI: 1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
• Clé InChI: MHMNJMPURVTYEJ-UHFFFAOYSA-N

Description

Description générale

In immuno histology/ cytology, Fluorescein-5-isothiocyanate (FITC) for biochemistry and for fluorescence microscopy, serve as marker substance for the visualization and for the detection of tissue-bound bio molecules like e.g. antigens, lectins, various proteins, peptides, nucleic acids, oligo- and polysaccharides in samples of human origin. FITC-marked antibodies bind specifically to the appropriate target structures in the tissue. An optimum FITC-protein (antibody) ratio is important for a good result.

Application

for biochemistry and for fluorescence microscopy

Remarque sur l'analyse

Dye content (spectrophotometrically): ≥ 95 %
Identity (UV/VIS-Spectrum): passes test
Absorption maximum λ max (Sodium hydroxide solution 0.1 mol/l): 488 - 494 nm
Spec. Absorptivity A 1%/1cm (λ_max; 0.003 g/l; NaOH 0.1 mol/l): 2200 - 2380
TLC-Test: passes test

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H317 - H334
• Conseils de prudence: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Classification des risques: Resp. Sens. 1 - Skin Sens. 1
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable