F7250

Fluorescein isothiocyanate isomer I

≥90% purity (HPLC), powder

• Synonyme(s) : FITC, Fluorescein 5-isothiocyanate
• Formule empirique (notation de Hill): C₂₁H₁₁NO₅S
• Numéro CAS: 3326-32-7
• Poids moléculaire : 389.38
• Numéro Beilstein : 1407295
• Numéro CE : 222-042-0
• Numéro MDL: MFCD00005063
• Code UNSPSC : 12171500
• ID de substance PubChem : 24894933
• Nomenclature NACRES : NA.47

Propriétés

• product name: Fluorescein isothiocyanate isomer I, suitable for protein labeling, ≥90% (HPLC), powder
• Niveau de qualité: 200
• Pureté: ≥90% (HPLC)
• Forme: powder
• Couleur: orange to dark orange
• Pf: >360 °C (lit.)
• Solubilité: acetone: 1 mg/mL
• Fluorescence: λ_ex 492 nm; λ_em 518 nm (green)
• Adéquation: suitable for protein labeling
• Application(s): diagnostic assay manufacturing; hematology; histology
• Température de stockage: 2-8°C
• Chaîne SMILES: Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1
• InChI: 1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
• Clé InChI: MHMNJMPURVTYEJ-UHFFFAOYSA-N

Description

Description générale

Fluorescein isothiocyanate (FITC) is yellow-orange in color with an absorption maximum at 495nm. Upon excitation, it emits a yellow-green color with an emission maximum at 525nm.
It is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins.
Fluorescein isothiocyanate isomer I has been proposed as a contact sensitizer.

Application

Reagent for the FITC labeling of proteins; microsequencing of proteins and peptides (HPLC)
Biological applications include use as a fluorescent labeling reagent for proteins, a fluorescent reagent for protein tracing, and a reagent in the fluorescent antibody technique for the rapid identification of pathogens. It may be employed as the derivatization reagent for amphetamine, methamphetamine, 3,4-methylenedioxymethamphetamine and P-phenylethylamine in human urine during their capillary electrophoretic (CE) determination. It may be used for the preparation of fluorescent antibodies. It was employed for in vitro sensitization studies.

Réactif pour le marquage FITC des protéines; le microséqueçage de protéines et de peptides (HPLC)

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H317,H334
• Conseils de prudence: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Classification des risques: Resp. Sens. 1 - Skin Sens. 1
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: dust mask type N95 (US), Eyeshields, Faceshields, Gloves