Accéder au contenu
Merck
Toutes les photos(1)

Key Documents

05-1245

Sigma-Aldrich

Anti-O-GlcNAc Antibody, clone 9D1.E4(10)

clone 9D1.E4(10), from mouse

Synonyme(s) :

O-GlcNAc transferase p110 subunit, O-GlcNAc transferase subunit p110, O-linked GlcNAc transferase, O-linked N-acetylglucosamine (GlcNAc) transferase

(UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase, O-linked N-acetylglucosam

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(1)

About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

100

Forme d'anticorps

purified antibody

Type de produit anticorps

primary antibodies

Clone

9D1.E4(10), monoclonal

Espèces réactives

human

Technique(s)

western blot: suitable

Isotype

IgG1

Numéro d'accès NCBI

NP_858058.1

Numéro d'accès UniProt

O15294

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

glycosylation

Informations sur le gène

human ... OGT(8473)

Description générale

O-linked N-acetylglucosamine (O-GlcNAc), is a member of the O-GlcNAc transferase family. O-GlcNAc is a posttranslational modification characterized by the attachment of N-acetylglucosamine to specific serine/threonine hydroxyl groups. It plays an important role in nutrient sensing, gene expression, and protein degradation. O-GlcNAc is widely expressed in the pancreas and is present in smaller amounts in skeletal muscle, heart, brain and placenta.

Spécificité

This antibody recognizes O-GlcNAc.

Immunogène

SANM-conjugated linear peptide corresponding to human O-GlcNAc.

Application

Detect O-GlcNAc using this Anti-O-GlcNAc Antibody, clone 9D1.E4(10) validated for use in WB.
Research Category
Signaling
Research Sub Category
General Post-translation Modification

Qualité

Evaluated by Western Blot in O-GlcNAc transferease transfected HEK293 cell lysate and O-GlcNAcase transfected transfected HEK293 cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected O-GlcNAc on 10 µg of
O-GlcNAc transferease transfected HEK293 cell lysate and O-GlcNAcase transfected transfected HEK293 cell lysate.

Description de la cible

Various

Forme physique

Format: Purified
Protein G Purified
Purified mouse monoclonal antibody in 0.1M Tris-Glycine (pH7.4) 150mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Maintain refrigerated at 2-8°C for up to 12 months.

Remarque sur l'analyse

Control
HEK293 cell lysate

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Vous ne trouvez pas le bon produit ?  

Essayez notre Outil de sélection de produits.

En option

Réf. du produit
Description
Tarif

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Glucose deprivation-induced increase in protein O-GlcNAcylation in cardiomyocytes is calcium-dependent.
Zou, L; Zhu-Mauldin, X; Marchase, RB; Paterson, AJ; Liu, J; Yang, Q; Chatham, JC
The Journal of Biological Chemistry null
Characterization of the specificity of O-GlcNAc reactive antibodies under conditions of starvation and stress.
Reeves, RA; Lee, A; Henry, R; Zachara, NE
Analytical biochemistry null
Glycopeptide-specific monoclonal antibodies suggest new roles for O-GlcNAc.
Teo, Chin Fen, et al.
Nature chemical biology, 6, 338-343 (2010)
Yuko Tashima et al.
The Journal of biological chemistry, 289(16), 11132-11142 (2014-02-28)
The transfer of N-acetylglucosamine (GlcNAc) to Ser or Thr in cytoplasmic and nuclear proteins is a well known post-translational modification that is catalyzed by the O-GlcNAc transferase OGT. A more recently identified O-GlcNAc transferase, EOGT, functions in the secretory pathway

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique