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906506

Sigma-Aldrich

HLAM-AβIδ1MεLVproSTγ-13CH3 Methyl Labeling Kit

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About This Item

Code UNSPSC :
12352200
Nomenclature NACRES :
NA.12

Technique(s)

bio NMR: suitable

Niveau de qualité

200

Conditions d'expédition

dry ice

Température de stockage

−70°C

Description générale

HLAM-AβIδ1MεLVproSTγ-13CH3 kit has 13CH3 isotopomer precursors and contains protocol instructions for creation of isotopically-labeled proteins.

Application

For detection of long-range nOes or for the NMR study of large proteins
HLAM-AβIδ1MεLVproSTγ-13CH3 is an optimized kit used to produce proteins with 13CH3 isotopomer on methyl groups of alanine, isoleucine, methionine, leucine and threonine residues. This kit has been tested with protein isotopic labeling in E. coli. It can be used to study large molecular system and complex protein structure in NMR spectroscopy along with other spectroscopic approaches. The sensitivity of the isotopomer can be enhanced by three-fold multiplicity. This kit can also be used to simplify crowded spectra to provide specific probes of dynamic and structural studies in solid state NMR spectroscopy.

Conditionnement

This product may be available from bulk stock and can be packaged on demand. For information on pricing, availability and packaging, please contact Stable Isotopes Customer Service. jj

Pictogrammes

Corrosion
GHS05

Mention d'avertissement

Danger

Mentions de danger

H314

Classification des risques

Skin Corr. 1B

Code de la classe de stockage

8A - Combustible corrosive hazardous materials

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Certificats d'analyse (COA)

Lot/Batch Number

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Consulter la Bibliothèque de documents

César Fernández et al.
Current opinion in structural biology, 13(5), 570-580 (2003-10-22)
Transverse relaxation-optimized spectroscopy (TROSY), in combination with various isotope-labeling techniques, has opened avenues to study biomolecules with molecular masses of up to 1000000Da by solution NMR. Important recent applications of TROSY include the structure determination of membrane proteins in detergent
Silke Wiesner et al.
Current opinion in structural biology, 35, 60-67 (2015-09-26)
Intermolecular interactions are indispensible for biological function. Here we discuss how novel NMR techniques can provide unique insights into the assembly, dynamics and regulation of biomolecular complexes. We focus on applications that exploit the methyl TROSY effect and show that

Articles

Methyl Isotope-Labeling of Proteins from NMR-Bio

Methyl groups are ideal probes for studying protein structure and function by NMR. Choose among kits for isotope labeling of a single type or multiple types of methyl groups.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

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