905410
TissueFab® bioink
Alg(Gel)ma -UV/365 nm
Synonyme(s) :
GelMA-alginate bioink
About This Item
Produits recommandés
Description
0.2 μm sterile filtered
suitable for 3D bioprinting applications
Forme
viscous liquid
Conditionnement
1 ea of 10 mL
Impuretés
≤5 CFU/g Bioburden (Fungal)
≤5 CFU/g Bioburden (Total Aerobic)
Couleur
colorless to pale yellow
pH
6.5-7.5
Application(s)
3D bioprinting
Température de stockage
2-8°C
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Description générale
Application
Conditionnement
Autres remarques
- Optimize printing conditions (e.g., nozzle diameter, printing speed, printing pressure, temperature, cell density) for the features of your 3D printer and your application.
- Reduce bubble formation. Air bubbles in bioink may hamper bioprinting. Carefully handle the bioink when you mix and transfer it to avoid bubble formation. Do not vortex or shake vigorously.
- UV light Crosslinking. Position the light source directly above the printed structure. Lower intensity light sources will require shorter distances and longer exposure times to complete crosslinking. Recommended conditions: Place an 800 mW/cm2 light source 8 cm above the printed structure and expose for 30 to 60 s.
Procedure
1. Prepare bioink solution: Warm TissueFab® - GelAlg-UV bioink in a water bath or incubator set to 37 °C for 30 minutes or until the bioink becomes fluid. Gently invert the bioink to make a homogeneous solution. DO NOT vortex or shake vigorously.
2. Prepare bioink-cell solution: Resuspend the cell pellet at the desired cell density with the bioink solution by gently pipetting up and down. Typical cell density for extrusion-based bioprinting is 1 to 5 x 106 cells/mL. Load the bioink-cell solution into the desired printer cartridge.
3. Bioprint: Cool the filled printer cartridge below 23 °C to induce gelation, using a temperature controlled printhead or place the cartridge at 4 °C for a few minutes. If print bed temperature control is available, set temperature to 20 °C. Follow the 3D printer manufacturer′s instructions. Load the print cartridge onto the 3D printer and print directly onto a Petri dish or into multi-well plates. Adjust the flow according to nozzle diameter, printing speed, printing pressure, and temperature. For optimal results, print under a gentle flow of 200 mM CaCl2 solution. A portable humidifier may be used to maintain the flow of the CaCl2 solution.
4. Crosslink: To photocrosslink, place the UV light source directly above the 3D-bioprinted structure and expose the structure to UV light (wavelength 365 nm). Use the appropriate distance settings and exposure times for your bioprinter. To chemically crosslink the printed structure, add 100mM CaCl2 in PBS for 1 minute. Rinse with PBS twice.
5. Culture cells: Culture the bioprinted tissue with appropriate cell culture medium following standard tissue culture procedures.
Informations légales
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
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Bioinks can be 3D bioprinted into functional tissue constructs for drug screening, disease modeling, and in vitro transplantation. Choose the Bioinks and method for specific tissues engineering applications.
Professor Shrike Zhang (Harvard Medical School, USA) discusses advances in 3D-bioprinted tissue models for in vitro drug testing, reviews bioink selections, and provides application examples of 3D bioprinting in tissue model biofabrication.
Professor Shrike Zhang (Harvard Medical School, USA) discusses advances in 3D-bioprinted tissue models for in vitro drug testing, reviews bioink selections, and provides application examples of 3D bioprinting in tissue model biofabrication.
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