MAIPNTR10
Multiscreen® 96 well Plate, hydrophobic PVDF membrane
pore size 0.45 μm, non-sterile
Synonym(s):
96-Well PVDF Plate, Hydrophobic 96-Well Plate, PVDF 96-Well Plate
About This Item
Recommended Products
material
PVDF membrane (hydrophobic)
acrylic
flat bottom wells
Quality Level
description
This clear 96 well filter plate has a Hydrophobic PVDF membrane used for PAMPA.
sterility
non-sterile
product line
MultiScreen®
feature
hydrophobic
lid
manufacturer/tradename
MultiScreen®
parameter
50-250 μL sample volume (per well)
technique(s)
parallel artificial membrane permeation assay (PAMPA): suitable
plate size
96 wells
well maximum volume
300 μL
working volume
50-250 μL
matrix
Immobilon®-P
pore size
0.45 μm pore size
binding type
high binding surface
shipped in
ambient
General description
MultiScreen Permeability, MultiScreen-IP, and MultiScreen Acceptor plates support artificial membrane strategies for predicting passive transport.
Membranes have uniform pore structure and density for reliable transport:
- Individual wells minimize candidate cross-talk
- Compatibility with automated liquid handling systems increases throughput
Permeability Assay:
- The MultiScreen Permeability plate has a polycarbonate membrane, which supports a hexane/hexadecane artificial layer for permeability assays.1
PAMPA Assay:
- The MultiScreen-IP plate has a hydrophobic PVDF membrane, which supports a lipid bilayer for PAMPA asays.2,3
MultiScreen Acceptor Plate:
- The MultiScreen Acceptor plate can be used with either plate to capture passively transported compounds. Constructed of 100% PTFE, the plate can improve LC/MS results depending on the charge of the small molecules being evaluated.
Application
Assays
Protein
Physical form
Other Notes
Legal Information
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Articles
This is a white paper on the Evaluation of the reproducibility of Parallel Artificial Membrane Permeation Assays (PAMPA) using MultiScreen® Filter Plates.
This is a protocol for a Membrane Integrity test for Lipid-PAMPA Artificial Membranes
This article details the benefits of combining solubility and a non-cell based permeability assay (Parallel Artificial Membrane Permeation Assay; PAMPA) into a single workflow in which a portion of the filtrate from the MultiScreen® Solubility filter plate is added to the donor compartment of the MultiScreen filter plate for the PAMPA analysis.
Protocols
HDM-PAMPA with the MultiScreen Permeability Filter Plate
Guidelines for various bioassays on the MultiScreen system, which makes it possible to carry out an assay from sample incubation to scintillation counting in a 96-well plate.
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