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Supelco

Ascentis® Express 90 Å RP-Amide (2.7 μm) HPLC Columns

L × I.D. 5 cm × 4.6 mm, HPLC Column

Synonym(s):

Core-shell (SPP) Fused Core polar embedded HPLC column

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

Ascentis® Express RP-Amide, 2.7 μm HPLC Column, 2.7 μm particle size, L × I.D. 5 cm × 4.6 mm

material

stainless steel column

Agency

suitable for USP L60

product line

Ascentis®

feature

endcapped

manufacturer/tradename

Ascentis®

packaging

1 ea of

parameter

60 °C temp. range
600 bar max. pressure (9000 psi)

technique(s)

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

L × I.D.

5 cm × 4.6 mm

surface area

135 m2/g

impurities

<5 ppm metals

matrix

Fused-Core® particle platform
superficially porous particle

matrix active group

amide, alkyl phase

particle size

2.7 μm

pore size

90 Å

operating pH range

2-9

application(s)

food and beverages

separation technique

reversed phase

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Application

UHPLC Analysis of Sunscreens on Ascentis® Express RP-Amide, 2.7 μm

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

Application

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guard cartridge

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related product

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required but not provided

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Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
USVH002047
USVH002046
USVH002045
USVH002044
USVH002043

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S Fan et al.
Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment, 30(12), 2093-2101 (2013-10-22)
A liquid chromatography-linear ion-trap spectrometry (LC-MS³) method using β-receptor molecular-imprinted polymer (MIP) solid-phase extraction (SPE) as clean-up was developed to determine simultaneously and confirmatively residues of 25 β₂-agonists and 21 β-blockers in urine samples. Urine samples were subjected to enzymatic
Michael T Furlong et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 926, 36-41 (2013-04-02)
Clopidogrel is prescribed for the treatment of Acute Coronary Syndrome and recent myocardial infarction, recent stroke, or established peripheral arterial disease. A sensitive and reliable high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) assay was developed and validated to enable reliable
Hirotaka Kushida et al.
Biomedical chromatography : BMC, 27(12), 1647-1656 (2013-07-03)
Uncaria Hook (UH) alkaloids are involved in the beneficial effects of Yokukansan. However, the pharmacokinetics of UH alkaloids after oral administration of Yokukansan has not yet been sufficiently investigated. Therefore, we developed and validated a sensitive and specific high-performance liquid
Fan Sai et al.
Journal of agricultural and food chemistry, 60(8), 1898-1905 (2012-02-15)
A sensitive method has been developed for the simultaneous determination of residues of 25 β₂-agonists and 23 β-blockers in animal foods by high-performance liquid chromatography coupled with linear ion trap mass spectrometry (HPLC-LIT-MS). This method is based on a new
Erzsébet Oláh et al.
Journal of chromatography. A, 1217(23), 3642-3653 (2010-04-23)
Today sub-2 microm packed columns are very popular to conduct fast chromatographic separations. The mass-transfer resistance depends on the particle size but some practical limits exist not to reach the theoretically expected plate height and mass-transfer resistance. Another approach applies
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Articles

Ascentis® Express RP-Amide HPLC Columns

Sigma-Aldrich.com presents an article about innovative particle technology and RP-Amide bonding chemistry provide the benefits of speed, resolution, selectivity, aqueous stability, and improved peak shape for bases.

Synthetic Cannabinoid Analysis

A large variety of cannabinoid drug standards, along with an HPLC method with the Supelco Ascentis® Express RP-Amide column, are presented in this article.

Ascentis® Express RP-Amide Columns for HPLC and UHPLC

Improve your separation of basic compounds with our Ascentis® Express RP-Amide HPLC columns from the Supelco® portfolio.

Protocols

HPLC Analysis of Bisphenol A, Dimethylbisphenol A and Nonylphenol on Ascentis® Express RP-Amide (UV Detection)

This application demonstrates the suitability of Ascentis Express C18 for the efficient separation of milk thistle related compounds

Chromatograms

application for HPLCapplication for HPLC

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