MABN621

Anti-Pan-Neurofascin Antibody, clone L11A/41

clone L11A/41, from mouse

• Synonym(s): Neurofascin
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: L11A/41, monoclonal
• species reactivity: rat
• technique(s): immunohistochemistry: suitable; western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_001153785
• UniProt accession no.: P97685
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: rat ... Nfasc(116690)

Description

General description

Neurofascin is a ankyrin binding protein that may be involved in myelination, neurite extension, synaptogenesis, axonal guidance and neuron-glial cell interactions. There are two known isoforms, NF155 and NF186. NF155 is found in oligodendrocytes, and is involved in neurite outgrowth and neuronal adhesion. NF186 is expressed in neurons, and may optimize communication between neurons by anchoring the axon initial segment (AIS).

Specificity

Other Homologies: Human (99% sequence homology).

Immunogen

Recombinant protein corresponding to rat Pan-Neurofascin.

Application

Detect Neurofascin using this mouse monoclonal antibody, Anti-Pan-Neurofascin Antibody, clone L11A/41 validated for use in western blotting & IHC.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Pan-Neuorfascin in rat pons tissue.

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

Quality

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Pan-Neurofascin in 10 µg of rat brain tissue lysate.

Target description

~185 kDa and ~155 kDa observed. Three isoforms are known to exist at 138 kDa, 134 kDa, and 132 kDa due to alternative splicing. This protein has been observed at ~186 kDa, ~180 kDa, ~166 kDa and ~155 kDa (Kriebel, M., et al. (2012). Int J Biochem Cell Biol. 44(5):694-697.). This protein may undergo post-translational modification.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Rat brain tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable